指状拟舟虫诱导牙鲆抗血清免疫球蛋白分析

对引起牙鲆体表溃烂的原纤毛虫－指状舟虫诱导牙鲆免疫反应产生的免疫球蛋白ＩｇＭ进行分析，结果表明，病原纤毛虫免疫注射牙鲆 ，可诱导牙鲆发生特异性免疫反应，产生抗体型上鲆抗指状拟舟虫血清的凝集试验，发现纤毛虫停止游动并发虫体聚集；抗血清经与标准分子量和鼠ＩｇＭ单克隆抗体以及对照血清的ＳＤＳ－ＰＡＧＥ电泳比较分析证明，牙鲆抗指状拟舟虫血清免疫球蛋白ＩｇＭ重链分子量为７１０００，轻链分子量为２３０００；Ｉ

Analysis of immunogloblin M (IgM) in serum of Japanese flounder, Paralichthys olivaceus

The immune response of Japanese flounder(Paralichthys olivaceus)against antigen of Paralembus digitiformis was detected. Agglutination test and SDS-PAGE were used respectively to investigate the serum antibody response in flounder injected with frozen-killed ciliates. The sera from immunized fish with the ciliated protozoon, Paralembus digitiformis, immobilized the parasite and caused the parasites agglutinated in vitro by agglutination test. The antiserum was analyzed by SDS-PAGE and proved that IgM had produced in the serum of immunized flounder. The molecular weights of the 2 subunits (H-chain and L-chain) of Japanese flounder IgM were estimated 71 000 and 23 000 respectively, by SDS-PAGE with 2-mercaptoethanol. The molecular weight of Japanese flounder IgM was determined to be 752 000.