达氏鲟实时荧光定量PCR内参基因的筛选

实时荧光定量PCR（qRT-PCR）是基因表达研究的常用方法，筛选达氏鲟（肌动蛋白（）、18S核糖体RNA（）共7个常用内参基因作为候选基因，利用geNorm、NormFinder、BestKeeper和RefFinder 4个软件分析这7个候选基因在达氏鲟成鱼不同组织和不同发育时期胚胎及性腺的表达稳定性。结果表明，7个候选内参基因的定量PCR引物均可获得特异性扩增产物和理想的扩增效率。在成鱼不同组织中，候选内参基因稳定性由高到低的顺序为18S rRNA > > GAPDH > -actin > ；在不同发育时期卵巢中，候选内参基因稳定性由高到低的顺序为 > ，而不同发育卵巢最稳定表达的内参基因是。本研究旨在为今后达氏鲟不同组织、不同发育时期胚胎及性腺的基因表达差异研究提供理论基础。

Identification of reference genes for qRT-PCR in Dabry's sturgeon, Acipenser dabryanus

In order to identify the most stable reference gene for the real-time quantitative PCR (qRT-PCR) analysis in Dabry''s sturgeon (), seven commonly used reference genes, including beta actin (), glyceraldehyde 3-phosphate dehydrogenase (18S rRNA), succinate dehydrogenase complex subunit A () were selected as candidates. Their expression stability was evaluated in different adult tissues, and in the tissue of embryos, testes, and ovaries during different developmental stages. We used geNorm, NormFinder, BestKeeper, and RefFinder software to conduct the analyses. Results verified that optimal amplification efficiency was obtained from the primers of these candidate genes. In adult tissues, the stability of these reference genes was as follows:18S rRNA > ; during different developmental stages of embryos, the stability of these reference genes was:18S rRNA > ; during different developmental stages of testes, the stability of these reference genes was:18S rRNA > ; during different developmental stages of ovaries, the stability of these reference genes was: > . Overall, the most stable reference gene in adult tissue, embryos, and testes was to be most stable. This study provides a useful basis for future work examining gene expression in Dabry''s sturgeon.