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栉孔扇贝核糖体DNA转录间隔子序列研究及其潜在应用
引用本文:喻子牛,孔晓瑜,庄志猛,刘亚军,宋林生.栉孔扇贝核糖体DNA转录间隔子序列研究及其潜在应用[J].中国水产科学,2001,8(1):6-9.
作者姓名:喻子牛  孔晓瑜  庄志猛  刘亚军  宋林生
作者单位:1. 青岛海洋大学教育部海水养殖重点实验室,山东青岛 266003
2. 中国水产研究院黄海水产研究所,山东青岛266071
3. 中国科学院海洋研究所,山东青岛 266071
基金项目:Supported by Grants of 973 Program (G1999012008);Natural Science Foundation of China (39600113 and 39620260).
摘    要:以相应引物PCR扩增栉孔扇贝(Chlamys farreri)核基因组的核糖体DNA两个转录间隔子(ITS-1和ITS-2),PCR产物经T载体连接后进行克隆、测序,分别得到了340bp和510bp的碱基序列,序列大小非常适合遗传变异及分子系统学研究。其A、T、G、C含量在ITS-1分别为32.06%,20.59%,22.35%和25.00%,在ITS-2分别为30.00%,21.37%24.12%和24.51%。这两个变异性较大的序列在扇贝种群中应用潜力很大,可广泛用于种内群体间遗传变异研究、种质鉴别及系统学研究。

关 键 词:栉孔扇贝  ITS-1  ITS-2  基因序列  核糖体DNA  转录间隔子
文章编号:1005-8737(2001)01-0006-04
修稿时间:2000年11月3日

Sequence study and potential uses of ribosomal DNA internal transcribed spacers in scallop Chlamys farreri
YU Zi niu ,KONG Xiao yu ,ZHUANG Zhi meng ,LIU Ya jun ,SONG Lin sheng.Sequence study and potential uses of ribosomal DNA internal transcribed spacers in scallop Chlamys farreri[J].Journal of Fishery Sciences of China,2001,8(1):6-9.
Authors:YU Zi niu  KONG Xiao yu  ZHUANG Zhi meng  LIU Ya jun  SONG Lin sheng
Institution:YU Zi niu 1,KONG Xiao yu 1,ZHUANG Zhi meng 2,LIU Ya jun 1,SONG Lin sheng 3
Abstract:The ribosomal internal transcribed spacer regions ITS-1 and ITS-2, extracted from mantle tissue of wild Chlamys farreri, were amplified via PCR using relevant primers. The PCR products were ligated into T-vector, then cloned and sequenced. Two nucleotide sequences were got with very suitable sizes of 340 bp and 510 bp for studies on genetic variation and phylogeny. The contents of A,T,G and C were 32.06%,20.59%,22.35% and 25.00% in ITS-1 and 30.00%, 21.37%, 24.12% and 24.51% in ITS-2. The primers of ITS-1 and ITS-2 proved to be very universal in a large variety of mollusc species. The potential uses of the 2 sequences for genetic variation studies and phylogenetic research in scallop species were discussed too.
Keywords:Chlamys farreri  ITS-1  ITS-2  nucleotide sequence
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