| 中华鳖同工酶研究 |
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| 引用本文： | 杨弘,夏德全,吴婷婷,简纪常,董在杰,曹萤,王涛.中华鳖同工酶研究[J].中国水产科学,1997,4(4):54-58. |
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| 作者姓名： | 杨弘 夏德全 吴婷婷 简纪常 董在杰 曹萤 王涛 |
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| 作者单位： | 中国水产科学研究院淡水渔业研究中心 |
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| 摘 要： | 采用垂直淀粉胶电泳对中华鳖脑、眼、心、肌、肝、肾和卵巢七种组织中的六种同工酶系统（ＬＤＨ、ＡＤＨ、ＩＤＨ、ＭＥ、Ｇ６ＰＤＨ、ＥＳＴ）进行研究，构建了中华鳖同工酶酶谱，并利用草鱼ＬＤＨ－Ｂ４抗体对中华鳖ＬＤＨ酶谱进行了免疫化学分析，确定了中华鳖ＬＤＨ同工酶酶谱和亚基组成，揭示了鱼类ＬＤＨ与爬行类ＬＤＨ在进化上的保守性
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| 关 键 词： | 中华鳖，同工酶，淀粉胶电泳，免疫化学反应 |
| 修稿时间： | 2010/4/8 0:00:00 |
STUDIES ON THE ENZYMES OF AMYDA SINENSIS |
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| Yang Hong,Xia Dequan,Wu Tingting,Jian Jichang,Dong Zaijie,Cao Ying,Wang Tao.STUDIES ON THE ENZYMES OF AMYDA SINENSIS[J].Journal of Fishery Sciences of China,1997,4(4):54-58. |
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| Authors: | Yang Hong Xia Dequan Wu Tingting Jian Jichang Dong Zaijie Cao Ying Wang Tao |
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| Abstract: | By use of vertical starch gel electrophoresis, the patterns of expression of six isozymes (LDH, IDH, ADH, ME, G6PDH and EST) were investigated in seven different tissues (brain, eye, heart, muscle, liver, kidney and ovary) of Amyda sinensis. The experession pattern of LDH was determinded by immunochmical analysis with antibody of LDH-B 4 of grass carp. The results suggested that LDH of fishes and reptiles is conservative in evolution. |
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| Keywords: | Amyda sinensis Isozyme Starch gel electrophoresis Immunochemical reaction |
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