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| 瓦氏雅罗鱼达里湖群体和乌苏里江群体的遗传多样性和遗传结构分析 | |
| 引用本文： | 池炳杰,常玉梅,闫学春,曹顶臣,高玉奎,柳玉海,李勇,梁利群.瓦氏雅罗鱼达里湖群体和乌苏里江群体的遗传多样性和遗传结构分析[J].中国水产科学,2010,17(2):228-235. |
| 作者姓名： | 池炳杰 常玉梅 闫学春 曹顶臣 高玉奎 柳玉海 李勇 梁利群 |
| 作者单位： | 1. 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;上海海洋大学,水产与生命学院,上海,201306 2. 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070 3. 赤峰市克什克腾旗达里诺日渔场,内蒙古,赤峰,024000 4. 黑龙江生物科技职业学院,水产系,黑龙江,哈尔滨,150025 |
| 摘 要： | 选用5个瓦氏雅罗鱼(Leuciscus waleckii Dybowski)微卫星标记和13个草鱼微卫星标记,对分别采自内蒙古境内的达里湖和乌苏里江抚远江段的各15尾野生瓦氏雅罗鱼个体进行STR-PCR分析。结果显示,18个微卫星标记共检测到111个等位基因,平均每个基因座扩增得到6.1667个等位基因,观测杂合度(H o)0.1000～0.9333,平均为0.5315;期望杂合度(H e)0.0966～0.9328,平均为0.6298;多态信息含量(PIC)0.0905～0.9113,平均为0.5774;乌苏里江群体的平均等位基因数和平均观测杂合度分别为5.5556和0.5778,达里湖群体的平均等位基因数和平均观测杂合度均低于乌苏里江群体的平均等位基因数和平均观测杂合度,分别为4.3889和0.4852。2个群体间的遗传距离(D c)为0.2550。个体聚类结果显示,达里湖雅罗鱼所有个体聚为一簇,乌苏里江雅罗鱼所有个体聚为一簇。群体间的遗传分化指数(F st)为0.0855,数值介于0.05～0.15,N m值为2.6749,表明这2个群体处于中等分化,存在一定程度的基因交流。对2个群体的基因型进行了Hardy-Weinberg平衡的卡方检验,结果表明两群体均在一定程度上偏离了平衡。 |
| 关 键 词： | 瓦氏雅罗鱼 微卫星标记 遗传多样性 遗传结构 |
| 修稿时间： | 2010/3/30 0:00:00 |
Genetic variability and genetic structure of Leuciscus waleckii Dybowski in Wusuli River and Dali Lake |
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| CHI Bingjie,CHANG Yumei,YAN Xuechun,CAO Dingchen,LI Yong,GAO Yukui,LIU Yuha,LIANG Liqun.Genetic variability and genetic structure of Leuciscus waleckii Dybowski in Wusuli River and Dali Lake[J].Journal of Fishery Sciences of China,2010,17(2):228-235. | |
| Authors: | CHI Bingjie CHANG Yumei YAN Xuechun CAO Dingchen LI Yong GAO Yukui LIU Yuha LIANG Liqun |
| Institution: | CHI Bingjie 1,2,CHANG Yumei 1,YAN Xuechun 1,CAO Dingchen 1,LI Yong 4,GAO Yukui 3,LIU Yuhai 3,LIANG Liqun 1 (1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.College of Fishery , Life Science,Shanghai Ocean University,Shanghai 201306,3.Dalinuori Fishery of Keshiketeng Banner,Chifeng 024000,4.Department of Aquaculture,Heilongjiang Vocational College of Biology Science , Technology,Harbin 150025,China) |
| Abstract: | Leuciscus waleckii Dybowski belongs to Cypriniformes,Cyprinidae and Leuciscus,mainly distributing in Amur River,Wusuli River,Songhua River,Liaohe River,Yellow River,Luanhe River and Nenjiang River.It is a cold water species and can survive in saline-alkaline waters above pH 9.0.While,a large number of L.waleckii survive in Dali Lake,which is located in the northeast of Inner Mongolia and is a saline-alkaline lake with a salinity of 6.96 and alkalinity of 53.57 mmol/L.Using 5 microsatellite markers of L.wale... |
| Keywords: | Leuciscus waleckii Dybowski microsatellite marker genetic diversity genetic structure |
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