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牙鲆抗鳗弧菌病AFLP分子标记筛选
引用本文:刘云国,陈松林,LIU,Zhan-jiang.牙鲆抗鳗弧菌病AFLP分子标记筛选[J].中国水产科学,2007,14(1):155-159.
作者姓名:刘云国  陈松林  LIU  Zhan-jiang
作者单位:1. 农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院,黄海水产研究所,山东,青岛,266071
2. Department of Fisheries and Applied Aquaculture,Auburn University,USA
基金项目:国家高技术研究发展计划(863计划);国家自然科学基金;青岛市科技将才计划项目
摘    要:牙鲆(Paralichthys olivaceus)感病群体和抗病群体通过注射鳗弧菌(Vibrio anguillarum)获得。利用61对AFLP引物组合扫描了牙鲆感病群体和抗病群体各20个个体,结果共扩增出3 200条带,8条AFLP带在2个群体中显示了极大的差异(P<0.01),其中有2条带是在抗病群体中出现的高显性基因频率的标记,另外6条带是在感病群体中出现的高显性基因频率的标记。这些标记很可能是与抗病性相关的候选标记。这些抗病性候选标记的获得为实现牙鲆分子标记辅助育种和抗病基因克隆奠定了一定基础。中国水产科学,2007,14(1):155-159]

关 键 词:牙鲆  鳗弧菌  AFLP标记  抗病力
文章编号:1005-8737-(2007)01-0155-05
收稿时间:2006-04-13
修稿时间:2006-07-24

Screening of AFLP markers for Vibrio anguillarum resistance in Japanese flounder
LIU Yun-guo,CHEN Song-lin,LIU Zhan-jiang.Screening of AFLP markers for Vibrio anguillarum resistance in Japanese flounder[J].Journal of Fishery Sciences of China,2007,14(1):155-159.
Authors:LIU Yun-guo  CHEN Song-lin  LIU Zhan-jiang
Institution:1. Key Laboratory for Sustainable Utilization of Marine Fishery Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Departmem of Fisheries and Applied Aquaculture, Auburn University, USA
Abstract:Japanese flounder(Paralichthys olivaceus) is a commercially important flat fish widely distributed throughout coastal areas of northern China.Market landings of this species have not increased greatly over the last decade in spite of extensive fishery management efforts,because viral and bacterial diseases plague Japanese flounder aquaculture industry all over the China.An important approach to prevent disease is to screen populations of fish that show innate genetic resistance to a certain disease.Susceptible and resistant populations of Japanese flounder were obtained by artificially challenged with Vibrio anguillarum.Twenty flounder individuals from the susceptible and resistant populations,respectively,were screened by 61 pairs of AFLP primers.A total of 3 200 fragments were obtained and eight showed significant dominant gene frequency differences(P<0.01)between susceptible and resistant groups,among which,two were in resistant gruop,six were in susceptible group.An efficient method for recovery of DNA fragment from denaturing polyacrylamide gels was developed to allow re-amplifying them.It is a basic work for the application of marker-assisted selection and cloning of anti-disease gene in Japanese flounder selective breeding program.Journal of Fishery Sciences of China,2007,14(1):155-159]
Keywords:Paralichthys olivaceus  Vibrio anguillarum  AFLP marker  disease-resistance
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