浒苔提取物对太平洋牡蛎受精卵孵化的抑制效应

针对2007-2009年间在黄海海域形成"绿潮".的大型绿藻浒苔(Enteromorpha prolifera).研究了其3种不同极性有机溶剂(甲醇、丙酮、氯仿)提取物对太平洋牡蛎(Crassostrea gigas)受精卵孵化的影响.结果表明.在48 h内.浒苔的甲醇和丙酮提取物对太平洋牡蛎受精卵孵化有较强的抑制作用.高浓度提取物能够完全抑制太平洋牡蛎受精卵孵化至D型幼虫.而氯仿提取物对太平洋牡蛎受精卵孵化的影响没有明显的浓度-效应关系.应用乙酸乙醋和石油醚对浒苔的甲醇提取物进行液-液萃取.并探讨了乙酸乙醋相和石油醚相中的浒苔提取物对牡蛎受精卵孵化的影响.结果表明.石油醚相和乙酸乙醋相中的浒苔提取物对牡蛎受精卵孵化均有很强的抑制效应.上述研究表明.浒苔中存在能够抑制太平洋牡tfi受精卵孵化的活性物质.这类物质应具有较高的极性.

Effects of Enteromorpha prolifera extract on hatchability of Pacific oyster(Crassostrea gigas) eggs

Enteromorph prolifera was the causative species of green tides in the Yellow Sea from 2007 to 2009. Three different organic solvents which were methanol, acetone and chloroform, were used to extract the dry tissue of , and the effects of extracts on hatchability of Pacific oyster) eggs were tested. It was found that the methanol and acetone extracts had strong inhibitory effects on hatching of oyster eggs, and their high-concentration extracts could completely inhibit the oyster eggs from developing into D-shaped larvae. The chloroform extract, however, did not show any significant concentration-dependent effects on the hatching rates of oyster eggs. The methanol extract of was further purified by liquid-liquid extraction with ethyl acetate and petroleum ether, and the ethyl acetate phase and petroleum ether phase were also tested for their effects on the hatching rates of oyster eggs. It was found that both ethyl acetate and petroleum ether extract could significantly inhibit hatching of oyster eggs. The results of this experiment suggest that can produce bioactive compounds inhibiting hatching of oyster eggs, and the compounds, probably with high polarity, can be extracted with methanol. However, the structure and characteristics of the bioactive compounds in need to be further studied.