近江牡蛎SLC13基因家族的全基因组鉴定及急性高盐胁迫下的表达特征

溶质载体13(solute carrier 13, SLC13)是SLC转运蛋白超家族的重要成员,编码结构相似的跨膜蛋白,在介导转运阴离子和柠檬酸循环代谢中间体中发挥重要作用。近江牡蛎(Crassostrea ariakensis)基因家族收缩和扩张分析表明,SLC13家族显著扩张,可能与渗透压调节密切相关。为进一步探讨近江牡蛎SLC13基因家族(CarSLC13)特征及在高盐胁迫下的表达变化,本研究运用生物信息学方法对CarSLC13进行鉴定,并分析了其基因结构、染色体定位、系统进化和在急性高盐胁迫后鳃组织中的表达特征。本研究共鉴定出11个CarSLC13基因,包括1个CarSLC13A1亚家族成员, 6个CarSLC13A2亚家族成员和4个CarSLC13A5亚家族成员,其中7个家族成员蛋白的理化性质较为稳定,不稳定系数均小于40;亚细胞定位预测显示,所有CarSLC13均定位到细胞膜或内膜;染色体定位结果显示, 11个SLC13基因定位在6条染色体上,在第3号染色体上的部分基因发生了串联复制;该基因家族成员都具有钠-硫酸盐共转运蛋白跨膜结构域(PF00939),该结构域与渗透压调...

Genome-wide identification of the SLC13 gene family in Crassostrea arakensis and its expression characteristics in gill under acute salt stress

The solute carrier 13 (SLC13) gene family is one of the members of super transporter families that commonly exist in eukaryotes and prokaryotes, and encodes transmembrane proteins with similar structures for transferring anions and citric acid cycle intermediates. Previous studies have shown that the SLC13 family significantly expanded in Crassostrea arakensis, suggesting that it might be closely related to salinity adaptation and osmotic pressure regulation. In this study, we identified the members of the CarSLC13 gene family and analyzed their gene structure, chromosome location, and phylogenetic relationship. In addition, qRT-PCR was used to detect the CarSLC13 mRNA expression at 6 h and 12 h after being exposed to acute salt stress of 40. A total of 11 CarSLC13 genes were identified, including one CarSLC13A1 member, six CarSLC13A2 members, and four CarSLC13A5 members. The physicochemical properties of these members, including molecular weight, theoretical pI, instability index, and hydropathicity, were also predicted. All CarSLC13 proteins were predicted to be localized to the cell membrane or inner membrane. The results of chromosome mapping showed that 11 SLC13 genes were located on six chromosomes, and some genes on chromosome 3 had tandem duplication. In addition, we speculated that this was related to the function of osmotic pressure regulation because all members of the CarSLC13 gene family had the same Na_sulph_Symp (PF00939) domain. The CarSLC13A2 and CarSLC13A5 subfamily genes in the gill tissues of C. arakensis generally increased first and then decreased after acute salt stress, and the expression level was the highest after 6 h of salt stress, while the relative expression of CarSLC13A1 subfamily genes decreased with time. This study clarified the characteristics, phylogeny, and response to high salt stress of the CarSLC13 gene family, enriching the research data of the SLC13 gene family in bivalves and providing a reference for further exploration of the role of the CarSLC13 gene family in osmotic pressure regulation.