饥饿及恢复生长对方斑东风螺抗氧化体系的影响

在(25.8±1.7)℃条件下,测定了方斑东风螺(Babylonia areolata)不同饥饿期(7d、15d、25d、40d)后再投喂(30d)过程中足肌、肝胰脏的抗氧化体系相关指标变化。结果显示,分别饥饿25d和40d时幼螺两组织丙二醛(MDA)含量较对照组均显著升高;饥饿25d前,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活力较对照组逐渐增强,当禁食达40d时却显著减弱;谷胱甘肽硫转移酶(GST)活力饥饿状态下先略上升后下降,肝胰脏、足肌中分别于饥饿25d和40d时显著降低;谷胱甘肽还原酶(GR)活力及谷胱甘肽(GSH)含量随禁食延长均呈下降趋势。恢复生长后,除饥饿40d组GR活力及肝胰脏MDA水平较对照呈差异显著外,该组其余指标及其他各处理组相应指标均恢复至或接近对照组水平。结果表明,饥饿胁迫下螺体抗氧化体系虽被激活,但仍处于一定的氧化应激状态,提示幼螺养成中禁食时间不宜超过25d,且SOD、CAT与GPx活性可作为螺体饥饿胁迫状态的指示参数。

Oxidative stress and antioxidant defenses during refeeding after starvation in tissues of juvenile spotted babylon,Babylonia areolata

An experiment was conducted to determine effects of refeeding after starvation on lipid peroxidation and antioxidant defenses of foot muscle and hepatopancreas of Babylonia areolata juveniles with initial body weight of (5.25±0.53) g at (25.8±1.7)℃. The results indicated that the malondialdehyde (MDA) content of the snails were significantly higher than that of the controls after food was deprived for 25 d and 40 d respectively. The activities of the antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were significantly higher than those in control in two tissues when food deprivation were not more than 25 d, but the results were reverse after the snails were starved for 40 d. The activities of glutathione-S-transferase (GST) reduced after increased firstly during starvation period, which were significantly lower than those in controls in muscle and hepatopancreas when starved time was up to 25 d and 40 d respectively. Both of the activities of glutathione reductase (GR) and the contents of GSH decreased gradually, with the starved time prolonged. After the snails were refed for 30 d, almost all the levels of the biomarkers of the snails returned to control values except MDA contents in hepatopancreas and GR activities in the 40 d starved group. In conclusion, prolonged starvation led to a pro-oxidant situation and oxidative stress despite activation of antioxidant defense mechanism, and the fasting time for the snails was best not more than 25 d during culture period when necessary. The activities of SOD, CAT and GPx could be used as good indexes to indicate the status of snails when food is deprived.