鳙小卫星pBC174的序列结构特性分析

用双脱氧DNA测序法对鳙 (Aristichthysnobilis)小卫星 pBC174进行序列测定。其长度为 1873bp。在这段序列中 ,A T含量高达 6 4 % ,从 15 11碱基处开始 ,含有多聚腺嘌呤 多聚胞嘧啶 ,即 (AC) 13 的短重复序列的隐蔽微卫星。序列中含有 2 0种限制性内切酶的 5 7个酶切位点 ,97个不同的正向重复序列 ,其中 71个 8bp、10个 9bp、10个 10bp、2个 11bp和 4个 17bp。根据序列分析 ,认为小卫星 pBC174是由富含A的重复序列TAAAGAAA和富含T的重复序列TTTTTACA等 2个不同的核心序列组成。

Analysis on sequence characters of Pbc174 OF BIGHEAD CARP Aristichthys nobilis

The minisatellite pBC174 of bighead carp (Aristischthys nobilis) was sequenced using Sanger method. Its size is 1 877 bp in length. The A T content is up to 64%. Within the pBC174,there is a cryptic microsatellite( AC) 13 at the 1 511 position,and 57 restriction endonuclease sites of 20 endonucleases exist in this sequence. The pBC174 contains 97 different direct repetitive sequences,among which there are 71 direct repeats of 8 bp, 10 of 9 bp,10 of 10 bp and 4 of 17 bp. According to sequence analysis, A rich repeat TAAAGAAA and T rich TTTTTACA are two different core sequences which constitute minisatellite pBC174.