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牙鲆和大菱鲆养殖群体的分子标记和遗传变异
引用本文:邹曙明,李思发,蔡完其.牙鲆和大菱鲆养殖群体的分子标记和遗传变异[J].中国水产科学,2000,7(4):6-9.
作者姓名:邹曙明  李思发  蔡完其
作者单位:农业部水产增养殖生态、生理重点开放实验室,上海水产大学,上海,200090
摘    要:用16个10碱基随机引物对养殖牙鲆(Paralichthys olivaceus)和大菱鲆(Acophthalmus maximus)进行基因组随机扩增多态性DNA(RAPD)遗传分析。发现:(1)多个引物具有种的特异性扩增带。其中引物S7扩增的690和1160bp带,引物S2扩增的680bp带为牙鲆的种的特异性谱带;而引物S7扩增的653和889bp带,引物S2扩增的650、870、990及11

关 键 词:牙鲆  大菱鲆PAPD标记  遗传变异  遗传多样性
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Molecular genetic markers and variations of cultured Paralichthys olivaceus and Scophthalmus maximus
ZOU Shu-ming,LI Si-fa,CAI Wan-qi.Molecular genetic markers and variations of cultured Paralichthys olivaceus and Scophthalmus maximus[J].Journal of Fishery Sciences of China,2000,7(4):6-9.
Authors:ZOU Shu-ming  LI Si-fa  CAI Wan-qi
Abstract:RAPD was employed to assess the genetic variations between 2 cultured Pleuronectformes species (Paralichthys olivaceus and Scophthalmus maximus). The results show that:(1)Two primers can amplify distinctive bands in 16 polymorphic 10-mer primers. The 690bp and 1 160bp bands amplified by S 7, and the 680bp band by S 2 are the character of P.olivaceus and the 653bp, 889bp bands by S 7, the 650bp, 870bp, 990bp, 1 161bp bands by S 2 are the character of S.maximus. (2) The average genetic similarity index and Shannon's index of phenotypic diversity within 2 species are 0.905 and 2.735 in P.olivaceus and 0.945 and 3.758 in S.maximus. The variances of average genetic similarity between individuals within each species were detected by SAS software.There was significant difference (P<0.05) among the species of P.olivaceus and no such character among the species of S.maximus.(3)Shannon's index of phenotypic diversity is 2.735 in P.olivaceus and 3.758 in S.maximus.
Keywords:Paralichthys olivaceus  Scophthalmus maximus  RAPD marker  genetic variation
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