首页 | 本学科首页   官方微博 | 高级检索  
     检索      

金乌贼同工酶分析
引用本文:韦柳枝,高天翔,张秀梅.金乌贼同工酶分析[J].中国水产科学,2005,12(5):549-555.
作者姓名:韦柳枝  高天翔  张秀梅
作者单位:中国海洋大学,教育部海水养殖重点实验室,山东,青岛,266003
基金项目:Supported by the Natural Science Foundation of Qingdao (04-2-JZ-80) and Key Technologies R&D Program of Shandong Province (2004GG2205110 ). We are grateful to Mr. Wei Bang-fu of Fishery Technical Extension Station of Rizhao for his enthusiastic assistance in collection of the samples. Thanks are also due to Liu Guang-dong, Liu Jin-xian, Han Zhi-qiang, Wan Zhen-zhen, Fang Hua-hua, Liu Man-hong and Huang Guo-qiang for their help in the experiment.
摘    要:采用水平淀粉凝胶电泳技术对金乌贼(Sepia esculenta)同工酶的组织特异性及其山东日照近海群体的遗传结构进行研究。对金乌贼眼、鳃、肌肉、口球、肝脏、鳃心6种组织的19种同工酶进行分析,检测出PGDH、GPI、MPI、IDHP、SOD、ME、AAT、DIA、MDH、LDH、G3PDH、PGM共12种同工酶在几种组织中有较稳定而清晰的表达。结果表明,金乌贼同工酶的表达有明显的组织特异性。选择金乌贼3种组织(眼、口球、鳃心)进行同工酶分析,共记录了18个基因座位,其中3个基因座位LDH-2^*、G3PDH-1^*、PGM^*呈多态,其多态座位比例为0.1667(P0.99)和0.0556(P0.95),平均观测杂合度和预期杂合度分别为0.0159和0.0143,平均有效等位基因数为1.0201,表明日照近海金乌贼群体的遗传多样性较低。

关 键 词:金乌贼  同工酶  组织特异性  遗传结构
文章编号:1005-8737-(2005)05-0549-07
收稿时间:2004-11-01
修稿时间:2005-03-14

Isozyme analysis of Sepia esculenta (Cephalopoda: Sepiidae)
WEI Liu-zhi,GAO Tian-xiang,ZHANG Xiu-mei.Isozyme analysis of Sepia esculenta (Cephalopoda: Sepiidae)[J].Journal of Fishery Sciences of China,2005,12(5):549-555.
Authors:WEI Liu-zhi  GAO Tian-xiang  ZHANG Xiu-mei
Abstract:Horizontal starch gel electrophoresis was used to investigate the tissue-specific and genetic structure of Sepia esculenta Hoyle,1885(Cephalopoda: Sepiidae).The specimens were collected from the coast of Rizhao,Shandong Province of China.A preliminary screening for 19 isozymes in six kinds of tissues(eye,branchia,mantle muscle,buccal bulb muscle,branchial heart,liver) was carried out using TC-7.0 buffer system.The results showed that the isozymes expression was highly tissue-specific in S.esculenta.Twelve isozymes(PGDH,GPI,MPI,IDHP,SOD,ME,AAT,DIA,MDH,LDH,G3PDH and PGM) and three kinds of tissues(eye,buccal bulb muscle and branchial heart) were selected for genetic analysis of S.esculenta population.Of the eighteen putative enzyme-coding loci examined,three loci were polymorphic,i.e.,LDH-2~*,G3PDH-1~* and PGM~*. The proportion of polymorphic loci was 0.166?7(P_(0.99)) and 0.055?6(P_(0.95)).The average values of observed and expected heterozygosity were 0.015?9 and 0.014?3,respectively.The average effective number of alleles was 1.020 1.Analysis of S.esculenta in the present study revealed low levels of genetic variability.
Keywords:Sepia esculenta  isozyme  tissue-specificity  genetic structure
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中国水产科学》浏览原始摘要信息
点击此处可从《中国水产科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号