| 珠江水系光唇裂腹鱼可渡河种群mtDNA D-loop序列多态性分析 |
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| 引用本文： | 韩虎峰,代应贵.珠江水系光唇裂腹鱼可渡河种群mtDNA D-loop序列多态性分析[J].中国水产科学,2010,17(1). |
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| 作者姓名： | 韩虎峰 代应贵 |
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| 作者单位： | 贵州大学,动物科学学院,贵州,贵阳,550025 |
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| 基金项目： | 国家自然科学基金项目，贵州省科学技术基金项目，贵州省省长基金项目]黔省专合字(2005)347] |
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| 摘 要： | 40尾光唇裂腹鱼(Schizothorax lissolabiatus)采自珠江水系可渡河,采用PCR扩增和直接测序技术对其mtDNA D-loop区481bp的序列进行了测定分析。该序列共计发现了10个变异位点,占分析总位点数的2.08%。定义了10种单倍型,单倍型多样度(Hd)为0.733,单倍型间平均遗传距离(P)为0.0051。核苷酸多样性(π)为0.0027,平均核苷酸差异数(K)为1.301。用单倍型间遗传距离构建的NJ树由2个支系组成。该研究显示光唇裂腹鱼可渡河种群mtDNA D-loop区多态性较低,表明光唇裂腹鱼可渡河种群遗传多样性贫乏,有必要采取综合措施开展光唇裂腹鱼可渡河种群的保护。
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| 关 键 词： | 光唇裂腹鱼 多态性 遗传多样性 |
Sequence polymorphism of mtDNA D-loop in the population of Schizothorax lissolabiatus from Kedu River in Pearl River system |
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| HAN Hufeng,DAI Yinggui.Sequence polymorphism of mtDNA D-loop in the population of Schizothorax lissolabiatus from Kedu River in Pearl River system[J].Journal of Fishery Sciences of China,2010,17(1). |
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| Authors: | HAN Hufeng DAI Yinggui |
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| Institution: | HAN Hufeng,DAI Yinggui(College of animal sciences,Guizhou University,Guiyang 550025,China) |
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| Abstract: | The sequence polymorphism of mtDNA D-loop in the Schizothorax lissolabiatus population in Kedu River was studied.Fourty individuals of the species were collected form Kedu River in Pearl River system.mt DNA D-loop sequence of 481 bp was analyzed by the methods of PCR and DNA sequencing.A total of 10 varable loci(2.08% of total loci) were detected in the sequence of mtDNA D-loop in the population.Ten haplotypes were detected from the 40 individuals.Haplotypic diversity(Hd) was 0.733,and average genetic dista... |
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| Keywords: | mtDNA D-loop Schizothorax lissolabiatus mtDNA D-loop polymorphism genetic diversity |
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