| 养殖刺参腐皮综合征 2 种致病菌间接荧光抗体快速检测方法 |
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| 引用本文： | 王印庚,谢建军,荣小军,廖梅杰,张正.养殖刺参腐皮综合征 2 种致病菌间接荧光抗体快速检测方法[J].中国水产科学,2010,17(2):329-337. |
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| 作者姓名： | 王印庚 谢建军 荣小军 廖梅杰 张正 |
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| 作者单位： | 1. 中国水产科学研究院,黄海水产研究所,青岛市海水鱼类种子工程与生物技术重点实验室,山东,青岛,266071
2. 浙江省海洋水产研究所,浙江,舟山,316100 |
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| 基金项目： | 国家863计划项目，山东省科技发展计划项目，青岛科技发展计划项目，山东省农业良种工程重大课题，青岛市公共领域科技支撑计划项目 |
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| 摘 要： | 以养殖刺参(Apostichopus japonicus)腐皮综合征的2种致病菌:灿烂弧菌(Vibrio splendidus)和假交替单胞菌(Pseudoalteromonas nigrifaciens)为抗原,分别制备兔抗血清。以载玻片为介质,建立了2种病原菌的间接荧光抗体检测技术(IFAT)。交叉反应、阻断试验和吸收试验结果均表明本方法特异性强。对人工感染实验中的养殖水体及发病刺参溃烂组织检测,可以检出水体和患病刺参溃烂组织中的相应病原菌,病原菌被染成明亮的黄绿色,检测灵敏度2.4×104cell/mL。冰冻切片检测结果显示,在刺参肿胀嘴部与溃烂肌肉处有大量染成黄绿色的细菌颗粒。结果表明:采用IFAT可以对刺参腐皮综合征2种致病菌进行准确快速的检测。该方法的建立对刺参腐皮综合征的流行病学调查及快速诊断具有重要意义。
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| 关 键 词： | 刺参 腐皮综合征 灿烂弧菌 假交替单胞菌 间接荧光抗体检测(IFAT) |
| 修稿时间： | 2010/3/29 0:00:00 |
Rapid detection of 2 pathogenses of skin ulcer syndrome in cultured Apostichopus japonicus by indirect fluorescence antibody technique |
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| WANG Yingeng,XIE Jianjun,Rong Xianjun,Liao Meijie,ZHANG Zheng.Rapid detection of 2 pathogenses of skin ulcer syndrome in cultured Apostichopus japonicus by indirect fluorescence antibody technique[J].Journal of Fishery Sciences of China,2010,17(2):329-337. |
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| Authors: | WANG Yingeng XIE Jianjun Rong Xianjun Liao Meijie ZHANG Zheng |
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| Institution: | WANG Yingeng 1,XIE Jianjun 2,Rong Xiaojun 1,Liao Meijie 1,ZHANG Zheng 1 (1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao Key Laboratory for Marine Fish Breeding , Biotechnology,Qingdao 266071,China,2.Marine Fishery Research Institute of Zhejiang Province,Zhoushan 316100,China) |
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| Abstract: | An indirect fluorescence antibody technique for the rapid detection of Vibrio splendidus and Pseudoalteromonas nigrifaciens-the two pathogens of the skin ulcer syndrome of sea cucumber Apostichopus japonicus, has been developed on glass slides.The antiserum of V.splendidus(KH1)and P.nigrifaciens(KH2)were prepared with rabbits.Cross-reactions of antisera with other 11 main marine pathogens were detected by agglutination,and the results showed low cross-reaction which was eliminated by heightening the dilutio... |
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| Keywords: | Apostichopus japonicus skin ulcer syndrome Vibrio splendidus Pseudoalteromonas nigrifaciens rapid detection indirect fluorescence antibody technique |
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