暗纹东方鲀精、卵超微结构及精子入卵早期电镜观察

本文对暗纹东方精、卵结构和精子早期入卵进行了电镜观察。观察结果：精子为鞭毛型结构，头部两端呈椭圆长柱形，核凹窝较深，颈部含８个线粒体，尾部微管束为“９＋２”结构，双尾丝裸露；卵为圆球形，卵膜表层有许多嵴、沟、微孔和一个受精孔；受精方式为单精或多精入孔入卵，单精受精。本文还对受精方式、人工繁殖技术等进行了讨论。

Ultrastructura observation on sperm egg and early sperm-penetration in Fugu obscurus

The ultrastructure of sperm and egg of Fugu obscurus and the early process of sperm-penetration were studied with electronic microscopy. The results show that the sperm is a type of flagellum,with a nucleus in its more-deeply sunken head and with 8 mitochondria in its neck;both ends of the head are in the shape of long elliptic column; the tail presents a 9 2axoneme structure with 2 naked tail fibers; the eggs are characterized by the ball-shape with many cristae,rills,micropores and a funnel-shaped micropyle on their surfaces.Studies on the fertilization revealed that fertilization may be in the patterns of single-sperm fertilization and single-sperm or multiple-sperm penetration.The way of fertilization and the technique of artificial reproduction are discussed.