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| 雄性黄颡鱼芳香化酶基因mRNA在性成熟期组织中表达分析 | |
| 引用本文： | 温海深,刘淼,祁保霞,何峰,李吉方,史丹,马瑞芹,胡建,母伟杰,张远青.雄性黄颡鱼芳香化酶基因mRNA在性成熟期组织中表达分析[J].海洋水产研究,2011,32(4):26-33. |
| 作者姓名： | 温海深 刘淼 祁保霞 何峰 李吉方 史丹 马瑞芹 胡建 母伟杰 张远青 |
| 作者单位： | 1. 中国海洋大学水产学院,青岛266003;农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,无锡214081 2. 中国海洋大学水产学院,青岛,266003 3. 内蒙古民族大学动物科学技术学院,通辽,028000 |
| 基金项目： | 农业部水生动物遗传育种和养殖生物学重点开放实验室重点课题(BZ200709)资助 |
| 摘 要： | 在水温分别为20(常温对照组)、25、30℃条件下,采用RT-PCR方法,研究性腺成熟期雄性黄颡鱼组织中P450芳香化酶(P450aromA和P450aromB)的mRNA表达水平,同时测定性腺成熟系数(GSI)、肝重指数(HSI)、肥满度(CF)和血浆睾酮(T),雌二醇(E2)含量。结果表明,P450aromA在性腺发育成熟期雄性黄颡鱼心脏、肝、脾、胃、肾脏、精巢、鳃、脑、头肾、肠管组织中均无表达,P450aromB只在脑和肠中有表达,在脑中表达量高于肠,表达量随着水温升高而显著下降;各组实验鱼血浆T和E2含量与芳香化酶基因表达量存在相关关系。该实验结果表明,P450aromA可能与精子发生相关,可能不参与黄颡鱼精子排放过程;P450aromB在雄鱼脑中高度表达,可能参与性腺成熟期精子活力保持与排放过程,且应激温度越高,对性腺成熟期精子活力保持与排放过程影响越大。 |
| 关 键 词： | 黄颡鱼 P450arom mRNA表达 睾酮 雌二醇 |
| 收稿时间： | 2010/11/3 0:00:00 |
| 修稿时间： | 2010/12/15 0:00:00 |
Analysis of P450arom gene mRNA expression inhistology of mature male Pelteobagrus fulvidraco |
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| WEN Hai-shen,LIU Miao,QI Bao-xia,HE Feng,LI Ji-fang,SHI Dan,MA Rui-qin,HU Jian,MU Wei-jie,ZHANG Yuan-qing.Analysis of P450arom gene mRNA expression inhistology of mature male Pelteobagrus fulvidraco[J].Marine Fisheries Research,2011,32(4):26-33. | |
| Authors: | WEN Hai-shen LIU Miao QI Bao-xia HE Feng LI Ji-fang SHI Dan MA Rui-qin HU Jian MU Wei-jie ZHANG Yuan-qing |
| Institution: | WEN Hai-shen1,2 LIU Miao1 QI Bao-xia3 HE Feng1 LI Ji-fang1SHI Dan1 MA Rui-qin1 HU Jian1 MU Wei-jie1 ZHANG Yuan-qing(1Fisheries College,Ocean University of China,Qingdao 266003)(2Key Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology,Ministry of Agriculture,Wuxi 214081) (3College of Animal Science and Technology,Inner Mongolia University for the Nationalities,Tongliao 028000) |
| Abstract: | The steady-state level of the P450arom(P450aromA and P450aromB) mRNAs of male Pelteobagrus fulvidraco cultured at different temperatures and growth conditions were analyzed by RT-PCR,and GSI,HSI,CF,serum sexual steroid hormones testosterone(T) and estradiol(E2) levels were measured by 125I radioimmunoassay(RIA).The spatial expression result showed that P450aromA mRNA was not present in ten tissues of male P.fulvidraco,including spermary,liver,stomach,intestines,gill,heart,spleen,kidney,head kidney,and brain... |
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