九孔鲍消化道及养殖水体中弧菌胞外毒力因子的分析

从汕尾健生鲍鱼养殖场成鲍养殖水体和消化道中分离筛选到22株弧菌,其中14株来自成鲍消化道,8株来自养殖水体。本文对这两种不同来源的菌株进行了致病因子(胞外酶及溶血毒素)的分析比较,同时采用API条带法对其进行了种类鉴定。结果发现,消化道中除1株溶藻弧菌和3株最小弧菌外,其余均为河流弧菌;而水体中除副溶血弧菌和创伤弧菌各1株外,其他也均为河流弧菌。实验还发现,在5株胞外酶分泌能力最强的弧菌中,只有Sh031株是副溶血弧菌,另外4株(Bh14、Sh02、Sh08、Sh05)均为的河流弧菌。结果显示,无论是养殖水体还是消化道,河流弧菌都应视为一种海水养殖贝类(鲍)的主要条件致病菌,是能力较强的胞外致病因子生产者。

Studies on extra-cellular pathogenic factors produced by vibrio from abalone digestion guts and culture waters

22 vibrio strains were isolated from mature abalone digestion guts and the culture water in Jiansheng Abalone Culture Farm of Shangwei, Guangdong province, among them, 14 strains were from abalone digestion guts and 8 strains were from culture water. The extra-cellular pathogenic factors produced by vibrio strains from the two sources were analyzed. Simultaneously, all the isolates were identified by API 20 E strips method. The results indicated that all the isolates from abalone digestion guts were V. fluvialis except 4 isolates which were identified as V. alginolyticus and V. minicus. As for the isolates from the culture waters, similarly, they were all identified as V. fluvialis except 2 isolates which were identified as V. parahaemolyticus and V. vulnificus. Besides, pathogenic factors analyses showed that among the five vibrio strains with the greatest capability of excreting extra-cellular enzymes, only one was V. parahaemolyticus, whereas other four were all V. fluvialis. Obviously, V. fluvialis should be regarded as a main pathogenic factor for the seawater culture abalone regardless of sources.