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牙鲆养殖群体遗传变异的微卫星标记研究
引用本文:刘云国,陈松林,李八方.牙鲆养殖群体遗传变异的微卫星标记研究[J].海洋水产研究,2005,26(5):27-33.
作者姓名:刘云国  陈松林  李八方
作者单位:1. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,青岛,266071;中国海洋大学,生命科学与技术学部,青岛,266003
2. 中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,青岛,266071
3. 中国海洋大学,生命科学与技术学部,青岛,266003
基金项目:国家863课题(2002AA626010)和国家自然科学基金项目(30413240)共同资助
摘    要:应用10对微卫星引物对中国牙鲆一个养殖群体的30个个体进行了群体遗传结构分析。结果显示,微卫星标记比其他标记具有更高的多态性,10个微卫星座位的等位基因数在4~10之间,有效等位基因数在2.23~5.82之间,平均等位基因数为7.6,群体平均杂合度为0.6960,Hardy-weinberg遗传偏离指数的平均值为0.1774。

关 键 词:牙鲆  养殖群体  遗传变异  微卫星标记
文章编号:1000-7075(2005)05-0027-07
收稿时间:2004-07-26
修稿时间:2005-05-15

Genetic structure analysis for the cultured stock of Japanese flounder(Paralichthys olivaceus) by microsatellite marker
LIU Yun-guo,CHEN Song-lin,LI Ba-fang.Genetic structure analysis for the cultured stock of Japanese flounder(Paralichthys olivaceus) by microsatellite marker[J].Marine Fisheries Research,2005,26(5):27-33.
Authors:LIU Yun-guo  CHEN Song-lin  LI Ba-fang
Institution:1. Key Laboratory for Sustainable Utilization of Marine Fishery Resources, MA, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2 .Division of Life Science and Technology, Ocean University of China, Qingdao 266003
Abstract:We examined population genetic structure using 10 microsatellite markers among 30 individuals of the cultured Japanese flounder stock. The results showed that the microsatellite marker was highly informative. All of the 10 microsatellite loci screened in this study showed marked polymorphism. The numbers of the alleles and effective alleles ranged from 4 to 10, and from 2.23 to 5.82, respectively. The average numbers of the allele and effective allele are 7.6 and 3.66. The average heterozygosity and average Hardy-weinberg departure value of the cultured flounder stock are 0. 696 0 and 0. 177 4, respectively.
Keywords:Japanese flounder(Paralichthys olivaceus) Cultured stock Microsatellite markers Genetic stracture analysis
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