两种养殖条件下牙鲆的血液生理生化指标比较分析

对池塘和室内水泥池养殖牙鲆的血液生理生化指标进行了比较研究,每种养殖条件下分别选取两种不同体重规格的牙鲆,即池塘小规格牙鲆（PSJF）、池塘大规格牙鲆（PBJF）、室内小规格牙鲆（ISJF）和室内大规格牙鲆（IBJF）作为研究对象.对全部试验鱼5项血液生理指标和13项血清生化成分进行了测定分析,结果显示,PSJF和PBJF的红细胞数量（RBC）均显著低于ISJF和IBJF（P＜0.05）,且前者红细胞沉降率（ESR）、红细胞脆性（EOF）、白细胞数量（WBC）及白细胞中淋巴细胞所占比例均显著高于后者（P＜0.05）.PSJF和PBJF的血清葡萄糖（GLU）、总蛋白（TP）、白蛋白（ALB）、球蛋白（GLO）、肌酐（CREA）、总胆固醇（CHOL）、甘油三酯（TG）浓度及碱性磷酸酶（AKP）活性均显著高于ISJF和IBJF（P＜0.05）,仅PSJF的乳酸脱氢酶（LDH）活性低于ISJF和IBJF（P＜0.05）.血清氯离子（Cl-）、钙离子（Ca2＋）浓度及谷草转氨酶（AST）、谷丙转氨酶（ALT）活性在各个试验组间均无显著差异（P＞0.05）.血液生理和生化指标的差异表明,池塘养殖牙鲆可能在呼吸代谢要求上低于室内水泥池养殖牙鲆,而在免疫水平和营养物质代谢水平上高于室内水泥池养殖牙鲆.

Comparative analysis of blood physiological and biochemical characteristics between pond- and indoor tank-culture of Japanese flounder Paralichthys olivaceus

Physiological and biochemical indices of blood from pond-and indoor tank-cultured Japanese flounder Paralichthys olivaceus were analyzed and compared in the present study.Four groups of fish at different body weights from pond-culture （PSJF,pond-cultured small Japanese flounder group,and PBJF,pond-cultured big Japanese flounder group） and indoor tank-cultured （ISJF,indoor tank-cultured small Japanese flounder group,and IBJF,indoor tankcultured big Japanese flounder group） were sampled respectively,then 5 blood physiological indices and 13 serum biochemical indices were determined.Results revealed that the amount of red blood cells （RBC） of PSJF and PBJF were significantly lower than that of ISJF and IBJF （P 〈0.05）,and erythrocyte sedimentation rate （ESR）,erythrocyte fragility （EOF）,the amount of white blood cells （WBC）,and lymphocyte proportion of leukocyte in blood were significantly higher in pond-culture fish （P〈0.05）.Besides,serum glucose（GLU）,total protein（TP）,albumin（ALB）,globulin （GLO）,creatinine （CREA）,cholesterol （CHOL）,triglyceride （TG）concentrations and alkaline phosphatase（AKP） activity of PSJF and PBJF were significantly higher than those in ISJF and IBJF （P〈0.05）.However,serum lactate dehydrogenase activity of PSJF was significantly lower than ISJF （P〈0.05）.Statistical analysis revealed no significant differences in serum chloride （Cl-） concentration,calcium （Ca2＋） concentration,alanine aminotransferase （ALT） activity and aspartate aminotransferase （AST） activity among all the groups （P 〉 0.05）.The results showed that pond-cultured Japanese flounder may have lower requirement for the respiratory metabolism than indoor tank-cultured Japanese flounder,whereas serum immunity level and nutrition metabolism level may be higher.These results may provide basic information on management of pond-cultured and indoor tank-cultured Japanese flounder.