| 中国对虾皮下及造血组织坏死杆状病毒（HHNBV）DNA探针的制备及应用 |
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| 引用本文： | 刘萍,张岩.中国对虾皮下及造血组织坏死杆状病毒（HHNBV）DNA探针的制备及应用[J].海洋水产研究,1995,16(1):59-62. |
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| 作者姓名： | 刘萍 张岩 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所 |
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| 摘 要： | ＨＨＮＢＶ是１９９３年中国对虾暴发性流行病的主要病原之一。从纯化的病毒中提取ＤＮＡ，用ＥｃｏＲｌ限制性内切酶酶切成ＤＮＡ片段，与ＰＵＣ１８体外重组，建立ＨＨＮＢＶＤＮＡ文库。从中筛选出三个重组质粒（５＃、８＃、９＃），它们所插入的片段大小分别为：２．８Ｋｂ、０．８Ｋｂ、１．６Ｋｂ，它们分别用光敏生物素标记成探针，与感染ＨＨＮＢＶ的病虾ＤＮＡ呈阳性反应，以此利用制备的ＨＨＮＢＶＤＮＡ探针的高敏感性和特异性来检测虾病。
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| 关 键 词： | 中国对虾 DNA探针 制备 斑点杂交 杆状病毒 |
THE RESEARCHES AND APPLICATIONS OF HHNBV DNA PROBES IN PENAEUS CHINENSIS |
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| Liu Ping,Zhang Yan,Kong Jie,Huang Jie,Wang Qing,Yang Conghai.THE RESEARCHES AND APPLICATIONS OF HHNBV DNA PROBES IN PENAEUS CHINENSIS[J].Marine Fisheries Research,1995,16(1):59-62. |
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| Authors: | Liu Ping Zhang Yan Kong Jie Huang Jie Wang Qing Yang Conghai |
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| Abstract: | HHNBV is one of the main causative pathezens for shrimp cliseazes occurred in 1993. Virual genomic DNA was extracted from HHNBV purified from infected shrimp.The DNA was digested by ECORl and partial DNA fragments were inserted into PUC18 plasmid. From those inserts,3 recombinants selected and the inserts, 2. 8kb,0.8kb and 1.6kb respctively were labelled by long arm light sensitive biotin and used as DNA probes. In dot blot test,these inserts could hybrid with infected shrimp and not with uninfected shrimps. |
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| Keywords: | Penaeus chinensis DNA probe HHNBV Dot Blot |
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