| 对虾病原菌2—5B菌株16SrRNA基因片段的克隆和序列测定 |
| |
| 引用本文： | 叶军,孔杰.对虾病原菌2—5B菌株16SrRNA基因片段的克隆和序列测定[J].海洋水产研究,1997,18(1):9-15. |
| |
| 作者姓名： | 叶军 孔杰 |
| |
| 作者单位： | 青岛海洋大学海洋生命学院!青岛266003(叶军，徐怀恕)，中国水产科学研究院黄海水产研究所!青岛266071(孔杰，刘萍，张岩，杨丛海) |
| |
| 摘 要： | 用引物ＰＬ１－ＰＬ２ＰＣＲ扩增对虾病原菌－坎普氏弧菌２－５Ｂ菌株１６ＳｒＲＮＡ基因－１２２３ｂｐ的片段，采用ｐＵＣ１９质粒构建ｄＴ载体法完成该片段的克隆。部分序列测定及分析结果表明，该菌株与ＧｅｎＢａｎｋ中坎普氏弧菌标准株序列之间同源性为９６．９４％。
|
| 关 键 词： | 对虾 病原菌 16SrRNA基因 克隆 序列 |
CLONING AND SEQUENCING OF 16 SrRNA GENE FRAGMENT FROM BACTERIAL PRAWN PATHOGEN STRAIN 2-5B |
| |
| Ye Jun.CLONING AND SEQUENCING OF 16 SrRNA GENE FRAGMENT FROM BACTERIAL PRAWN PATHOGEN STRAIN 2-5B[J].Marine Fisheries Research,1997,18(1):9-15. |
| |
| Authors: | Ye Jun |
| |
| Abstract: | A 1 223-bp fragment of 16SrRNA gene from strain 2-5B Vibrio camphelliiisolated from diseased prawn was amplified by using PL1-PL2 primer pair. The PCR productwas gel purified and cloned into dT-vector conducted with pU C 19 plasmid. The partial primary sequence data exhibited considerable high level of similarity value (96. 94 % ) with thesequence data of type strain from GenBank. The sequence may be used to design PCRprimers and specific oligonucleotide probes and used in molecular identification of bacterialPrawn pathogens. |
| |
| Keywords: | Prawn Bacterial pathogen 16SrRNA gene Sequence |
| 本文献已被 CNKI 维普 等数据库收录！ |
