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红鳍东方鲀养殖群体生化遗传变异初步研究
引用本文:陈艳翠,高天翔,陈四清,张岩.红鳍东方鲀养殖群体生化遗传变异初步研究[J].海洋水产研究,2007(4).
作者姓名:陈艳翠  高天翔  陈四清  张岩
作者单位:中国海洋大学海水养殖教育部重点实验室,中国海洋大学海水养殖教育部重点实验室,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所 青岛266003中国水产科学研究院黄海水产研究所,青岛266071,青岛266003,青岛266071,青岛266071
摘    要:采用水平凝胶电泳技术对红鳍东方鲀养殖群体进行了同工酶分析。本实验采用肌肉和肝脏两种组织,分析了8种酶,检测到9个位点,在0.99水平上,PGM*和IDHP*表现为多态,MPI*、LDH*、AAT*、SOD*、MDH-1*、MDH-2*和G3PDH*均为单态,多态位点比例为0.2222,平均预期杂合度为0.0998,实际观测杂合度为0.0467,平均有效等位基因数目为1.1840,PGM*位点的遗传偏离指数为-0.0707,杂合子缺失,IDHP*位点无杂合子,遗传偏离指数为-1。

关 键 词:红鳍东方鲀  养殖群体  遗传结构  同工酶分析

A preliminary study on genetic variation of cultured Takifugu rubripes population
CHEN Yan-cui, GAO Tian-xiang CHEN Si-qing ZHANG Yan.A preliminary study on genetic variation of cultured Takifugu rubripes population[J].Marine Fisheries Research,2007(4).
Authors:CHEN Yan-cui  GAO Tian-xiang CHEN Si-qing ZHANG Yan
Institution:CHEN Yan-cui1,2 GAO Tian-xiang1 CHEN Si-qing2 ZHANG Yan2
Abstract:Isozyme analysis was conducted on cultured Takifugu rubripes population by using horizontal starch gel electrophoresis. Nine loci were examined from eight enzymes, and PGMand IDHP were polymorphic when the most common allele was less than or equal to 0.99. The proportion of polymorphic was 0.222 2, the average observed heterozygosity was 0.046 7, and the expected heterozygosity was 0.099 8. The average effective number of alleles was 1.184 0.
Keywords:Takifugu rubripes Cultured population Genetic structureIsozyme analysis
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