对虾皮下及造血组织坏死杆状病毒单克隆抗体的研制

用经密度梯度离心纯化的对虾皮下及造血组织坏死杆状病毒（ＨＨＮＢＶ—９３７）注射Ｂａｌｂ／ｃ小鼠，取其免疫后的脾细胞与ｓｐ２／０骨髓瘤细胞进行融合。经筛选与克隆，获得７株抗ＨＨＮＢＶ的单克隆杂交瘤细胞株。其中Ｆ９株的染色体数为８７，ＥＬＩＳＡ测定培养上清中单抗滴度为１∶８，腹水为１∶１０００。患有杆状病毒的皮下及造血组织坏死症的对虾组织切片的单抗酶联免疫染色，表明Ｆ９株单抗只与ＨＨＮＢＶ病灶发生特异性结合，与对虾组织结构不发生特异性反应。将其用于现场样品的检测研究表明，该抗体适于用来进行对虾暴发性流行病的病原检测。

PREPARATION OF MONOCLONAL ANTIBODY AGAINST HYPODERMAL AND HEMATOPOIETIC NECROSIS BACULOVIRUS OF PENAEID SHRIMP

Balb/c mice were injected with HHNBV-937(Hypodermal and hematopoietic necrosis baculovirus) purified through density gradient centrifugation.The splenic cells of immunized mice were fused with myeloma cells SP2/0. After several selection and cloning, seven hybridomas were obtained. F9,one of positive hybridoma,has the following characteristics: chromosome 87, titre 1:8 in cell culture supernant and 1:1000 in ascites tested by indirect ELISA. More important, F9 only react with HHNBV foci rather than shrimp tissues when immunoperoxidase staining tests were made with histological sections of the shrimp with EEDS.Also,the monoclonal antibody is practical for field diagnosis of HHNBV.