溶藻弧菌对三疣梭子蟹溶菌酶和磷酸酶活性的影响

将健康的三疣梭子蟹（平均体重176±21.3g）分别注射生理盐水和溶藻弧菌,在感染后0、24、48和72h分别采集不同处理组梭子蟹的血淋巴、肌肉和肝胰腺组织,测定其中的溶菌酶（LSZ）、酸性磷酸酶（ACP）及碱性磷酸酶（ALP）活性,研究溶藻弧菌对三疣梭子蟹体内免疫相关酶活的影响。结果表明,感染组梭子蟹血淋巴LSZ活性在感染后24h显著高于对照组（P〈0.05）,随后活性显著降低（P〈0.05）;肌肉和肝胰腺组织中LSZ活性在感染24h有稍微上升的趋势,但是随着感染时间的延长其活性又逐渐下降,且在感染48h后分别显著低于对照组（P〈0.05）。感染组梭子蟹不同组织中ACP和ALP酶活随着感染时间的延长均呈下降的趋势,且感染48h后显著低于对照组（P〈0.05）。试验表明,三疣梭子蟹感染溶藻弧菌后对机体免疫相关酶活指标的影响较大,导致机体免疫防御能力的减弱。

Effects on lysozyme and phosphatase activities of Portunus trituberculatus infected by Vibrio alginolyticus

Healthy Portunus trituberculatus （averaging body weight 176±21.3g） were used to determine the changes of the immunity enzyme activities in haemolymph, muscle and hepatopancreas tissue after the crabs were injected with 0.9% Nacl solution （Control） and Vibrio alginolyticus. The relative immunity indicators were measured at Oh, 24h, 48h and 72h after the infection. The results showed that： The lysozyme （LSZ） activity of heamolymph increased significantly （P〈0.05） in V. alginolyticus infected crabs in the initial 24h, but at 48h and 72h the activity reduced significantly （P〈0.05） below the control level. The LSZ activities of muscle and hepatopancreas decreased significantly after infection （P〈0.05）. Both the ACP and ALP activities reduced significantly in the heamolymph, muscle and hepatopancreas tissue of infection group respectively （P〈0.05）. However, the control crabs did not experience any significant variation in LSZ, ACP and ALP activities in all the tissues. The results of this study indicated that immunity enzyme in P. trituberculatus infected by V. alginolyticu was induced more significantly, which accompanied by weakened immunity ability of the crabs.