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产海洋细菌MP-2酯酶菌株的鉴定及酯酶理化性质的研究
引用本文:平芮巾,孙谧,刘均忠,王跃军,郝建华,张胜军.产海洋细菌MP-2酯酶菌株的鉴定及酯酶理化性质的研究[J].海洋水产研究,2009,30(2):83-88.
作者姓名:平芮巾  孙谧  刘均忠  王跃军  郝建华  张胜军
作者单位:1. 中国水产科学研究院黄海水产研究所海洋酶与酶工程实验室,青岛,266071;大连水产学院,116023
2. 中国水产科学研究院黄海水产研究所海洋酶与酶工程实验室,青岛,266071
3. 青岛市环境保护监测站,266003
基金项目:国家自然科学基金,国际科技合作重点计划项目 
摘    要:从渤海海泥样品中分离获得1株新型酯酶菌株,经鉴定为地衣芽孢杆菌Bacillus licheniformis。所得的MP-2酯酶的最适作用温度范围50~70℃,在60℃表现出了最高活性,属于耐热酶;最适作用pH为10,属于碱性酶,其pH值作用范围比较窄;具有良好的热稳定性;金属离子Co2+,Li+对酶具有激活作用,Ca2+对酯酶的活力没有显著影响,化学试剂SDS、EDTA及Tween-20对酯酶的抑制效果显著,对常见有机溶剂具有良好的耐受力;该酯酶对碳链长短不同的底物表现出不同的酶活。

关 键 词:海洋地衣芽孢杆菌  酯酶  鉴定  性质
收稿时间:2007/5/22 0:00:00
修稿时间:2007/7/23 0:00:00

Identification of MP-2 esterase producing marine Bacillus and study on properties of the esterase
PING Rui-jin,SUN Mi,LIU Jun-zhong,WANG Yue-jun,HAO Jian-hua,ZHANG Sheng-jun.Identification of MP-2 esterase producing marine Bacillus and study on properties of the esterase[J].Marine Fisheries Research,2009,30(2):83-88.
Authors:PING Rui-jin  SUN Mi  LIU Jun-zhong  WANG Yue-jun  HAO Jian-hua  ZHANG Sheng-jun
Institution:1 Laboratory of Marine Enzyme and Enzyme Engineering;Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2 Dalian Fisheries University;116023;3 Environmental Monitoring Station of Qingdao;266003
Abstract:A novel marine esterase-producing bacteria isolated from the Bohai Sea sediment was identified as Bacillus licheniformis. The MP-2 esterase was primarily purified and characterized. The optimal range of temperature of the esterase was 50-70 ℃, and the maximum activity was achieved at 60 ℃ and pH10.0. The esterase was alkaline and its optimum range of pH was narrow. The thermal stability of the esterase was good. Co^2+,Li+ ions were found to activate the esterase, but Ca^2+ had no significant effects on the activity of the esterase. While SDS, EDTA and Tween-20 had significantly suppressive effects on the esterase. It showed strong resistance to ordinary organic solvents. The enzyme exhibited different esterase
activities towards substrates with different length of carbon chains.
Keywords:Marine Bacillus licheniformis Esterase Identification Characteristics  
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