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高效液相色谱法检测海水养殖环境中喹诺酮类药物残留
引用本文:张汉华.高效液相色谱法检测海水养殖环境中喹诺酮类药物残留[J].海洋水产研究,2010,31(2):95-101.
作者姓名:张汉华
作者单位:1. 中国水产科学研究院黄海水产研究所,青岛,266071;上海海洋大学水产与生命学院,201306
2. 中国水产科学研究院黄海水产研究所,青岛,266071
基金项目:国家科技支撑计划项目(2007BAD621305)资助
摘    要:建立了一种养殖海水中诺氟沙星、环丙沙星和恩诺沙星3种喹诺酮类抗生素残留量的高效液相色谱-荧光测定方法。水样经稀盐酸调pH后经HLB固相萃取柱富集、净化,用外标法定量。结果表明,诺氟沙星、环丙沙星和恩诺沙星检出限分别是2、1和1μg/L,定量限分别是6.6、3.3和3.3μg/L,添加浓度为10、20和50μg/L时,3种物质的回收率为71.9%~85.3%,批内变异系数≤10%,批间变异系数≤7%。利用该方法对黄海沿岸部分养殖场及近岸海水(北起36°40.303′N,121°8.939′E;南至35°39.36′N,119°52.433′E)进行分析,诺氟沙星、环丙沙星、恩诺沙星的检出质量浓度分别为6.20~982、55.2和11.6~55.4μg/L。实验结果表明,该方法灵敏度高,重现性好,适用于养殖海水中诺氟沙星、环丙沙星和恩诺沙星的检测。

关 键 词:喹诺酮类抗生素  高效液相色谱  固相萃取  海水  
收稿时间:2009/11/6 0:00:00
修稿时间:2009/12/1 0:00:00

Determination of quinolones drug residues in aquacultural seawater using HPLC method
YANG Shou-guo,LI Zhao-xin,WANG Qing-yin,GUO Meng-meng,TAN Zhi-jun,XING Li-hong,YAO Jian-hua.Determination of quinolones drug residues in aquacultural seawater using HPLC method[J].Marine Fisheries Research,2010,31(2):95-101.
Authors:YANG Shou-guo  LI Zhao-xin  WANG Qing-yin  GUO Meng-meng  TAN Zhi-jun  XING Li-hong  YAO Jian-hua
Institution:1Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071;2College of Fisheries and Life Science;Ocean University of Shanghai;201306
Abstract:A detection method of high-performance liquid chromatography (HPLC) coupled with fluorescence was developed for the determination of residues of three quinolones in seawater.pH of were water samples adjusted with dilute HCl followed by extraction and concentration through hydrophilic-lipophilic balance cartridges,and three antibiotic quinolone compounds were determined using external standard quantification method.The results showed that the detection limits of norfloxacin,ciprofloxacin and enrofloxacin wer...
Keywords:Quinolones  High performance liquid chromatography  (HPLC)  Solid phase extraction  Seawater
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