土霉素在黑鲷体内的药物代谢动力学研究

首次报道了黑鲷口服土霉素的药物代谢动力学特征，用高效液相色谱法测定组织中的药物含量，药物在肌肉，血液，肝脏中的平均回归率分别为８５.61%,85.38%,82.005,该方法的检测限可达0.01μg/g，黑鲷１次口服剂量为７５mg/kg的土壤素后，其血液药物浓度－时间数据符合一室开放动力学模型，吸收速率常数（ka)为０.296/h，达峰时间（Ｔamx)为１０.635h,峰浓度（Ｃmax)为１.398μg/ml, 分布半衰期（Ｔ１／２a）为２.339h ,消除半衰期(T1/20β）为46.663h，药时曲线下面积（ＡＵＣ）为１１0.25mg/L.h，黑绸口服药物０.5h后在血液，肥肉，肝脏，肾脏４种组织中就可以检测到药物的存在，药物在１６h的采样点浓度达最高，分别为1.68μg/ml,1.68,2.52,6.77μg/g。

Pharmacokinetic study of oxytetracycline in Black seabream

This article is the first to report Pharmacokinetics property ofOTC in Black seabream after oral administration. OTC concentration was determ ined by high efficient liquid chromatography.The result demonstrated: data of OT C concentrations versus time conformed to the open dynamic model of one compart ment after a single oral administration at a dose of 75mg/kg, Absorption rate constant （ka）was 0.296/h, Time to reach maximum concentration （Tmax） was 10.635h, Maximum concentration（Cmax）was 1.398μg/ml, Absorption half-life（T1/2α）was 2.339h, Elimination half-life（ T1/2β）was 46.663h, Area under serum concentration (AUC) was 110. 25mg/L.h, we could determine the concentration of OTC in four ti ssues (muscle, blood, liver and kinedy) after 0.5h for administration and the m aximum concentration was 1.68、1.68、2.52、6.77μg/g respectively at 16h after a dministration,Average recoveries were 85.61%、85.38%、82.00% respective ly in muscle,blood and liver, and the limit of determination was 0.01μg/g.