| 三疣梭子蟹4个野生群体遗传差异的同工酶分析 |
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| 引用本文： | 樊祥国,高保全,刘萍,李健.三疣梭子蟹4个野生群体遗传差异的同工酶分析[J].海洋水产研究,2009,30(4):84-89. |
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| 作者姓名： | 樊祥国 高保全 刘萍 李健 |
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| 作者单位： | 1. 中国海洋大学生命科学与技术学部,青岛,266003
2. 中国水产科学研究院,黄海水产研究所,青岛,266071 |
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| 基金项目： | 国家高技术研究发展计划项目"863"，国家自然科学基金项目，青岛市科技计划项目 |
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| 摘 要： | 采用聚丙烯酰胺不连续凝胶垂直电泳技术对三疣梭子蟹Portunus trituberbuculatus4个野生群体(鸭绿江口、莱州湾、海州湾和舟山)的同工酶进行检测,分析群体间的生化遗传差异.结果发现(1) 4个群体的多态座位百分数(P0.99)分别为:鸭绿江口13.6%、莱州湾13.6%、海州湾13.6%和舟山13.6%.(2) 4个群体间的生化遗传差异不显著,4个群体间的遗传距离D=0.000 54～0.001 70,属种内群体间差异.(3)聚类分析表明:4个群体分为两组,海州湾和舟山两群体之间的亲缘关系最近,聚为一组.莱州湾和鸭绿江口群体亲缘关系比较近,聚为另一组.
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| 关 键 词： | 三疣梭子蟹 野生群体 同工酶 遗传差异 |
| 收稿时间： | 2008/1/16 0:00:00 |
| 修稿时间： | 2008/3/29 0:00:00 |
Genetic variation analysis of four wild populations of Portunus trituberculatus by isozyme |
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| FAN Xiang-guo,GAO Bao-quan,LIU Ping,Li Jian.Genetic variation analysis of four wild populations of Portunus trituberculatus by isozyme[J].Marine Fisheries Research,2009,30(4):84-89. |
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| Authors: | FAN Xiang-guo GAO Bao-quan LIU Ping Li Jian |
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| Institution: | 1College of Life Science and Technology;Ocean University of China;Qingdao 266003;2Yellow Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Qingdao 266071 |
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| Abstract: | Vertical polyacrylamide gel electrophoresis was used to investigate the genetic variation of isozyme of Portunus trituberbuculatus. Four wild geographic populations distributed in the Yalujiangkou(YL),Laizhou Bay(LZ),Zhoushan(ZS) and Haizhou Bay(HZ) (48 individuals each )were surveyed. Of 22 loci encoding 11 enzymes,three were polymorphic. The results showed that: (1)the mean proportion of polymorphic loci(P0.99) of YL,LZ,ZS and HZ were 13.6%,13.6%,13.6% and 13.6%,respectively ;(2) there were no significant... |
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| Keywords: | Portunus trituberculatus Wild populations Isozyme Genetic diversity |
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