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产低温碱性蛋白酶黄海黄杆菌YS-9412-130高产菌株的选育
引用本文:张云波,孙谧,洪义国,王跃军,阎晓玲,董美娜,桑玉泉,田杰,王瑞娟.产低温碱性蛋白酶黄海黄杆菌YS-9412-130高产菌株的选育[J].海洋水产研究,2000,21(4):14-19.
作者姓名:张云波  孙谧  洪义国  王跃军  阎晓玲  董美娜  桑玉泉  田杰  王瑞娟
作者单位:1. 中国水产科学研究院黄海水产研究所 海洋酶与酶工程开放实验室, 青岛 266071
2. 青岛海洋大学海洋生命学院, 266003
基金项目:国家“海洋863”项目(863-819-06-01)资助
摘    要:以黄海黄杆菌YS-9412-130菌株为出发株,经亚硝基胍、硫酸二乙酯、紫外线(UV)与微波(MI)复合诱变和自然选育,获得1株产低温碱性蛋白酶高产稳定的突变株YS-9412-130-SW1-104,其产低温碱性蛋白酶为出发株的16倍。在摇瓶发酵培养条件下,酶活力达2320U/ml(20℃测定),该诱变株具有Ref^r、Str^r、Amp^r和Met^-、Lys^-标记。突变株与出发菌株在细胞形态上比较,结果表明二者没有显著差异。

关 键 词:低温碱性蛋白酶  黄海黄杆菌  诱变  选育
修稿时间:2000年11月4日

Studies on the screening of highly producing strains of F. yellowsea of low-temperature alkaline protease
ZHANG Yun-bo.Studies on the screening of highly producing strains of F. yellowsea of low-temperature alkaline protease[J].Marine Fisheries Research,2000,21(4):14-19.
Authors:ZHANG Yun-bo
Abstract:In screening high producer of low-temperature alkaline protease, F. yellowsea YS-9412-130 is chosen as original strain, which is treated repeatedly with various mutagens(NTG、DEM、UV and Microwave). The mutant YS-9412-130-SW1-104 with high and stable yield of alkaline protease is selected after repeated natural selection. The alkaline protease activity of this mutant is in the 16 fold of the original strain, reaching 2 320U/ml (assayed 20℃). The mutant has marked with Refr、Strr、Ampr 、Met- and Lys-.The result shows that there are no significant differences between the cells of the mutant and the original strain.
Keywords:Low  temperature alkaline protease      F  yellowsea  YS  9412  130    Mutagenesis  
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