共培养系统中4种微藻生态因子的研究

通过二次回归通用旋转组合设计安排实验，研究光强、温度和盐度对分离自对虾池塘水环境中的啮蚀隐藻、新月菱形藻、微绿球藻和蛋白核小球藻的增长率的影响。获得各微藻的最适生态因子及其影响度。啮蚀隐藻的最适生态因子为：光强5750～7944lX，温度21．3～28．3℃，盐度13．3～23．0；影响度依次为：盐度〉温度〉光强。新月菱形藻的最适生态因子为：光强5761～86971X，温度23．4～29．6℃，盐度11．9～25．7；影响度依次为：盐度〉光强〉温度。蛋白核小球藻的最适生态因子为：光强6754～8775iX，温度17．1～20．7℃，盐度19．6～26．4；影响度依次为：温度〉盐度〉光强。微绿球藻的最适生态因子为：光强7128～9012lX，温度18．7～26．7℃，盐度17．9～24．3；影响度为光强〉温度〉盐度。啮蚀隐藻和新月菱形藻的增长率受到以盐度为基础、以光强和温度为协同因子的显著影响。

Studies on the ecological factors of four microalgae populations in mixed-culture

By means of the common rotation composite design of quadratic regression, four mathematical models were established to analyze the impact of light intensity (x_1), temperature (x_2) and salinity (x_3) on cell division rate (k) of each microalgal species seperated from shrimp ponds in mixed-culture. The suitable conditions for the microalgae growth and the weightiness of each factor to the cell division rate were obtained. The suitable light intensity for Cryptomonas erosa ranged from 5 750 lx to 7 944 lx, the suitable temperatures ranged from 21.3 ℃ to 28.3 ℃ and the suitable salinity ranged from 13.3 to 23.0. The weightiness of the impact of each factor on cell division rate was x_3>x_2>x_1. The suitable light intensity for Nitzschia closterium ranged from 5 761 lx to 8 697 lx, the suitable temperatures ranged from 23.4 ℃ to 29.6 ℃ and the suitable salinity ranged from 11.9 to 25.7. The weightiness of the impact of each factor on cell division rate was x_3>x_1>x_2. The suitable light intensity for Nannochloropsis oculata ranged from 6754lx to 8775lx, the suitable temperatures ranged from 17.1 ℃ to 20.7 ℃ and the suitable salinity ranged from 19.6 to 26.4. The weightiness of the impact of each factor on cell division rate was x_2>x_3>x_1.The suitable light intensity conditions for Chlorella pyrenoidosa ranged from 7 128 lx to 9 012 lx, the suitable temperatures ranged from 18.7 ℃ to 26.7 ℃ and the suitable salinity ranged from 17.9 to 24.3. The weightiness of the impact of each factor on cell division rate was x_1>x_2>x_3. The k of Cryptomonas erosa and Nitzschia closterium was significantly affected by salinity acting synergistically with light intensity and temperature.