| 瓦氏黄颡鱼头肾组织学观察与先天性免疫屏障研究 |
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| 引用本文： | 陈杰,贺扬,戴旭平,王均,覃川杰,李锐.瓦氏黄颡鱼头肾组织学观察与先天性免疫屏障研究[J].南方水产科学,2021(1). |
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| 作者姓名： | 陈杰 贺扬 戴旭平 王均 覃川杰 李锐 |
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| 作者单位： | 内江师范学院生命科学学院;内江师范学院长江上游鱼类资源保护与利用四川省重点实验室 |
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| 基金项目： | 四川省科技计划应用基础研究项目(2018JY0286);内江师范学院大学生创新创业孵化项目(X2019033)。 |
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| 摘 要： | 为探明瓦氏黄颡鱼(Pelteobagrus vachelli)头肾组织结构和先天性免疫屏障,该研究用苏木素-伊红(HE)染色法和改良James染色法对瓦氏黄颡鱼头肾的显微结构进行了观察,并使用台盼蓝活体染色法对头肾免疫屏障部位进行了定位。结果显示,瓦氏黄颡鱼头肾由被膜、淋巴细胞聚集区、粒细胞聚集区、前肾间组织、网状纤维和胶原纤维组成,胶原纤维仅在血管外周可见;台盼蓝主要分布于血窦周围的内皮细胞和一些游离的巨噬细胞中,且随时间延长呈递增趋势。研究表明血窦周围的内皮细胞和巨噬细胞组成了瓦氏黄颡鱼的头肾先天性免疫屏障。
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| 关 键 词： | 瓦氏黄颡鱼 头肾 组织结构 免疫功能 |
Histological observation and innate immune barrier study of head kidney of Pelteobagrus vachelli |
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| CHEN Jie,HE Yang,DAI Xuping,WANG Jun,QING Chuanjie,LI Rui.Histological observation and innate immune barrier study of head kidney of Pelteobagrus vachelli[J].South China Fisheries Science,2021(1). |
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| Authors: | CHEN Jie HE Yang DAI Xuping WANG Jun QING Chuanjie LI Rui |
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| Institution: | (College of Life Science,Neijiang Normal University,Neijiang 641000,China;Sichuan Key Laboratory of Fish Resources Protection and Utilization in the Upper Reaches of the Yangtze River,Neijiang Normal University,Neijiang 641000,China) |
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| Abstract: | To investigate the histological structure and immune function of head kidney of Pelteobagrus vachelli,we used hematoxylin-eosin(HE)staining and modified James staining to observe its microscopic structure,and applied trypan blue vital staining to locate its immune barrier site.The results show that the head kidney of P.wallichii consisted of capsule,lymphoid zone,granulocytic zone,anterior interrenal tissue,reticular fibers and collagen fibers,and collagen fibers were only visible at the periphery of blood vessels.Trypan blue was mainly distributed in endothelial cells around sinusoids and some free melanin macrophages,showing an increasing trend with time.Results suggest that endothelial cells of red pulps and free macrophages form the innate immune barrier of head kidney. |
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| Keywords: | Pelteobagrus vachelli Head kidney Tissue structure Immune function |
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