台盼蓝活体染色法观察瓦氏黄颡鱼血脾屏障研究

为探讨瓦氏黄颡鱼(Pelteobagrus vachelli)脾脏组织结构和血脾屏障位置,该研究对其脾脏进行了形态大小、组织学和活体染色观察。结果显示,瓦氏黄颡鱼的脾脏位于腹腔中部,呈暗红色,脾体指数为0.120%~0.273%;对健康脾脏制备石蜡切片,分别进行HE染色和改良James染色,显微镜观察发现其脾组织主要由不发达的被膜系统、分界不清的红髓和白髓及由网状纤维和胶原纤维组成的纤维支架构成。其中网状纤维主要位于脾索和椭球体周围,胶原纤维主要位于脾索;使用台盼蓝活体染色法对健康瓦氏黄颡鱼的血脾屏障部位和功能探索发现,台盼蓝主要位于脾脏的椭球体内皮细胞中,最早出现在注射后的第4小时,随时间推移在椭球体内逐渐累积,24 h后数量显著增加,并持续增加至第72小时。结果表明椭球体内皮细胞是血脾屏障的重要组成成分。

Location of blood-spleen barrier of Pelteobagrus vachelli by Trypan blue staining

To explore the structure of the spleen of Pelteobagruvs vachelli and the location of its blood-spleen barrier,we observed its spleen in morphology,size,histology and in vivo staining.The results show that the spleen of P.vachelli was located in the middle of the abdominal cavity with dark red colour,and the spleen body index was 0.120%–0.273%.Paraffin sections of healthy spleen were prepared and stained with HE and modified James,and the microscopic observation reveals that the spleen tissue was mainly composed of underdeveloped capsule system,unclear boundary between red and white pulp,and fibers that were composed of reticular fibers and collagen fibers.Reticular fibers were mainly located around the splenic cord and ellipsoid;collagen fibers were mainly located in the splenic cord.We applied Trypan blue in vivo staining method to explore the position and function of the bloodspleen barrier,and found that Trypan blue was mainly located in the ellipsoid endothelial cells of the spleen,and first appeared at 4^thhour after injection.It accumulated in the ellipsoid gradually along with time,and the number increased significantly after 24 h,and continued to increase until 72^ndhour.It is shown that the ellipsoid endothelial cells are an important component of the blood-spleen barrier.