乙酰甲喹对小新月菱形藻、等鞭金藻3011的毒性效应

通过考察乙酰甲喹对小新月菱形藻(Nitzschia closterium f.minutissima)、等鞭金藻(Isochrysis galbana Parke3011)的生长抑制和对叶绿素a、总超氧岐化酶(T-SOD)活性以及丙二醛(MDA)浓度的影响,研究了乙酰甲喹对2种微藻的毒性效应,为评价兽药乙酰甲喹的使用安全性和保护养殖环境提供基础数据。结果表明,乙酰甲喹对2种微藻的生长抑制作用随质量浓度的增加而增大,乙酰甲喹对小新月菱形藻的24 h-EC50(半数有效浓度)为4.73 mg·L~(-1)、对等鞭金藻3011的24 h-EC50为2.14 mg·L~(-1),乙酰甲喹对2种微藻均属于高毒物质,等鞭金藻3011更敏感。暴露24 h后,随着乙酰甲喹质量浓度的升高,小新月菱形藻叶绿素a浓度下降而等鞭金藻3011叶绿素a浓度基本不受影响;2种微藻的T-SOD活性随着乙酰甲喹质量浓度的升高均显著增加,各实验组MDA浓度也高于对照组,等鞭金藻3011更敏感,说明乙酰甲喹对2种微藻均能造成氧化胁迫,对细胞造成氧化损伤。

Toxic effect of mequindox on Nitzschia closterium f. minutissima and Isochrysis galbana Parke 3011

We studied the toxic effect of mequindox on growth inhibition,chlorophyll a content,total superoxide dismutase (T-SOD) activity and malondialdehyde (MDA) content of Nitzschia closterium f. minutissima and Isochrysis galbana Parke 3011,so as to provide basic data for safety evaluation of mequindox and protection of aquaculture environment. The results show that mequindox had acute toxicity effect on the growth of the two marine microalgae. The 24 h-EC50 values of mequindox to N.closterium f. minutissima and I.galbana Parke 3011 were 4.73 mg·L-1 and 2.14 mg·L-1,respectively. The I.galbana Parke 3011 was more sensitive. After 24-hour exposure of mequindox,the chlorophyll a content of N.closterium f. minutissima decreased with increasing mequindox concentration significantly but no adverse effect was found on I.galbana Parke 3011. The T-SOD activity increased significantly with increasing mequindox concentration and the MDA content in all the experimental groups were higher than that in the control group,and I.galbana Parke 3011 was more sensitive. It is suggested that mequindox can cause oxidative stress and oxidative damage to cells of the two marine microalgae.