卵形鲳鲹Tf、TNFα和C-Lys的组织分布及对美人鱼发光杆菌感染的响应

研究了卵形鲳鲹(Trachinotus ovatus)中免疫相关因子转铁蛋白(Tf)、肿瘤坏死因子α(TNFα)、C-型溶菌酶(C-Lys)的组织表达分布,并分析了这3种基因在人工感染美人鱼发光杆菌杀鱼亚种(Photobacterium damselae subsp.piscicida)后在卵形鲳鲹肾脏中的表达变化,探讨其对美人鱼发光杆菌感染的反应。结果显示,感染后第48小时卵形鲳鲹累计死亡率最高(76.4%);卵形鲳鲹肾脏中细菌数量从第3~第96小时一直处于下降趋势,从1.07×106CFU·g~(-1)下降到1.29×103CFU·g~(-1)。健康卵形鲳鲹中Tf和TNFα在肝脏中表达量最高,CLys在头肾中表达量最高。美人鱼发光杆菌感染后卵形鲳鲹肾脏中Tf表达量在第3~第12小时实验组高于对照组,其中第6小时的表达量最高,是对照组的17.99倍;TNFα在感染后的第3、第6、第24小时实验组的表达量高于对照组,第24小时组相对于对照组的表达量最高,是对照组的6.05倍。C-Lys在感染后感染组表达量均低于对照组。以上结果表明,美人鱼发光杆菌感染能促进卵形鲳鲹肾脏组织中Tf、TNFα的表达,而抑制C-Lys的表达。

Tissue distribution and response to infection of Photobacterium damselae subsp.piscicida of Tf,TNFα and C-Lys in Trachinotus ovatus

The study investigates the tissue expression of transferrin (Tf),tumor necrosis factor alpha (TNFα) and C-type lysozyme (C-Lys) in healthy Trachinotus ovatus after it was infected with Photobacterium damselae subsp.piscicida in kidney.The results show that after infection,the accumulative mortalities of T.ovatus reached the maximum in 48 h (76.4%);and the colony of P.damselae in T.ovatus' kidney continued to decline in 3~96 h from 1.07×10.6 CFU·g-1to 1.29×10.3 CFU·g-1.The Tf and TNFα expressions were the highest in the liver,and the C-Lys expression was the highest in the head kidney in healthy T.ovatus.After TOS1 infected,the Tf expression in 3~12 h in test group was higher than that of control and was the highest in 6 h (17.99 times as high as that of control).The TNFα expression of the test group was higher than that of the control after infection of 3 h,6 h and 24 h.The TNFα expression was the highest in 24 h (6.05 times higher than that of control).The C-Lys expression in infection group was lower than that in control in 3~96 h.The study indicates that the Tf and TNFα expressions can be promoted while C-Lys will be suppressed after TOS1 infection in T.ovatus' kidney.