Direct contact between Pseudo-nitzschia?multiseries and bacteria is necessary for the diatom to produce a high level of domoic acid |
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Authors: | Kenji Kobayashi Yoshinobu Takata and Masaaki Kodama |
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Institution: | (1) School of Marine Biosciences, Kitasato University, Ofunato Iwate, 022-0101, Japan |
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Abstract: | Pseudo-nitzschia multiseries loses most of its ability to produce domoic acid when it is cultured axenically. However, domoic acid production recovers
when bacteria from the original culture are reintroduced to axenic cultures, indicating a bacterial association with domoic
acid production in this species. In order to determine the role of bacteria in domoic acid production, an axenic strain prepared
from a nonaxenic strain of P. multiseries was inoculated into media in cellophane tubes, which were then placed in a bottle containing the original nonaxenic culture.
Both strains showed characteristic domoic acid production similar to that previously observed. Domoic acid level in the cells
of the axenic strain in the tubes was much smaller than that of the cells outside the tubes. These results show that direct
contact between living bacteria and P. multiseries is necessary for producing high levels of domoic acid in P. multiseries. |
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Keywords: | Co-culture of axenic and nonaxenic strains Domoic acid Pseudo-nitzschia multiseries |
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