Morphological changes in gill mitochondria-rich cells in cultured Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> acclimated to a wide range of environmental salinity |
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Authors: | Mi Young Seo Kyung Mi Lee Toyoji Kaneko |
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Institution: | (1) Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan; |
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Abstract: | Morphological changes in gill mitochondria-rich (MR) cells were examined in cultured Japanese eel acclimated to deionized
freshwater (DFW), freshwater (FW), 30%-diluted seawater (DSW), and seawater (SW). The gill Na+/K+-ATPase activity was higher in SW-acclimated eel than in those acclimated to DFW, FW, and DSW. Immunocytochemical observations
revealed that MR cells in the gill filaments were most developed in SW, whereas MR cells in the lamellae were preferentially
observed in DFW, suggesting that filament and lamellar MR cells are responsible for ion secretion and absorption, respectively.
In scanning electron microscopic observations, the apical membrane of lamellar MR cells appeared as a flat or slightly projecting
disk with a mesh-like structure on its surface. In contrast, the apical membrane of filament MR cells showed a slightly concave
surface. Whole-mount immunocytochemistry revealed that most MR cells showed cystic fibrosis transmembrane conductance regulator
immunoreaction in their apical region in fish in DSW and SW, but not in those in DFW and FW, indicating that MR cells developed
in DSW and SW function as an ion-secreting site. In addition to MR cells, distinct Na+/K+-ATPase immunoreaction was observed in the outermost layer of gill epithelia, suggesting that pavement cells are an additional
site of ion uptake in the gills. |
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