Muscle fiber types,growth and development in the whole myotome of cultured Pacific bluefin tuna Thunnus orientalis |
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Authors: | Roy Bimol Chandra Ando Masashi Nakatani Masahiro Okada Tokihiko Sawada Yoshifumi Itoh Tomohiro Tsukamasa Yasuyuki |
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Institution: | (1) Laboratory of Aquatic Food Science, Department of Fisheries, Faculty of Agriculture, Kinki University, Nara 631-8505, Japan;(2) Fisheries Laboratory, Kinki University, Wakayama 649-5145, Japan |
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Abstract: | Following the successful development of Pacific bluefin tuna (PBT) aquaculture, it is of considerable importance to determine
the muscle fiber types and their growth patterns for future development. Muscle fiber profiles of dorsal ordinary, lateral
ordinary (LO) and dark muscles and their growth patterns in PBT from 3.0 to 54.3 kg body weight were studied. Muscle fibers
were histochemically stained for NADH-diaphorase and myosin adenosine triphosphatase activity (mATPase), and immunohistochemically
stained with S-58 slow-muscle myosin antibody. All muscle fibers in dorsal and LO muscles showed low NADH-diaphorase activity,
and acid-labile (pH 4.0 or 4.3) and alkali-stable mATPase activity. In LO muscle adjacent to dark muscle, three intensities
of mATPase activity were observed after acid pre-incubation at pH 4.5 or 5.0, and the activity was related to the muscle fiber
diameter. In dark muscle, all small and some large fibers stained intensely for NADH-diaphorase activity, related to their
high aerobic metabolism. The high-active fibers with NADH-diaphorase in dark muscle were positive for S-58 antibody. Some
large fibers in dark muscle showed intermediate NADH-diaphorase activity and high mATPase activity after alkali pre-incubations.
These are fast-twitch oxido-glycolytic fibers in dark muscle and transformed to red muscle fibers with increasing body weight. |
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