Species identification method for marine products of Seriola and related species |
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Authors: | Jun Iguchi Yasuharu Takashima Atsushi Namikoshi and Michiaki Yamashita |
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Institution: | (1) Food and Agricultural Materials Inspection Center, 2-1 Shintoshin, Chuo-ku, Saitama 330-9731, Japan;(2) Food and Agricultural Materials Inspection Center, Kobe Regional Center, 1-3-7 Minatoshima-minamimachi, Chuo-ku, Kobe 651-0047, Japan;(3) National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236-8648, Japan |
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Abstract: | The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S.
rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially
important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphe
japonica, and Rachycentron
canadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific
primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the
PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a
373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species. |
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