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Genetic divergence of kingfish from Japan, Australia and New Zealand inferred by microsatellite DNA and mitochondrial DNA control region markers
Authors:Estu Nugroho  Douglas J Ferrell  Peter Smith  Nobuhiko Taniguchi
Institution:Department of Environmental Bioremediation, Graduate School for Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan;, New South Wales Fisheries Research Institute, Cronulla, New South Wales 2230, Australia and; National Institute of Water &Atmospheric Research Ltd, Wellington, New Zealand
Abstract:ABSTRACT: Genetic polymorphism in kingfish, collected from coastal waters of Japan, Australia and New Zealand, were examined using microsatellite (MS) DNA and mitochondrial DNA (mtDNA) control region markers. Sixteen to 25.7 alleles per locus were observed in three MS markers, while the average observed (and expected) heterozygosities were 0.782 (0.918), 0.750 (0.809) and 0.650 (0.888) for Australian, Japanese and New Zealand kingfish, respectively. Twelve mtDNA haplotypes were detected by the digestion of control region sequences with five endonucleases: Hae III, Hinf I Mbo I, Rsa I and Taq I. Significant genetic divergence was observed between the kingfish population from Japan and those from Australia–New Zealand. There was no significant differentiation among the Australian and New Zealand population samples.
Keywords:genetic divergence  kingfish  microsatellite DNA  mitochondrial DNA control region              Seriola lalandi
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