Isolation of microsatellite markers by in silico screening implicated for genetic linkage mapping in Japanese pufferfish Takifugu rubripes |
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| Authors: | Satoshi FURUKAWA Hirohiko TAKESHIMA Taro OTAKA Toru MITSUBOSHI Kunio SHIRASU Daisuke IKEDA Gen KANEKO Mutsumi NISHIDA AND Shugo WATABE |
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| Institution: | Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657;, Ocean Research Institute, The University of Tokyo, Nakano, Tokyo 164-8639;and Nissui Oita Marine Biological Technology Center, Tsurumi, Oita 876-1204, Japan |
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| Abstract: | ABSTRACT: The search for dinucleotide repeat microsatellites within scaffolds 1–25 of genome database JGI Fugu v3.0 for the pufferfish Takifugu rubripes revealed that 80% of microsatellite loci consisted of five to 13-fold repeats with locus-specific differences in density. Eleven out of 15 microsatellite loci isolated from the database with which genotyping using wild pufferfish was successfully performed showed polymorphism; that is, the means of the number of alleles and expected and observed heterozygosities at these 11 loci were 21.8, 0.915 and 0.829, respectively. It was confirmed that eight out of the 11 polymorphic loci were inherited through the Mendelian law and one pair of microsatellite loci derived from the same scaffold was linked. These results demonstrated that these loci are useful for constructing a linkage map in the pufferfish as DNA markers. |
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| Keywords: | fugu genome database linkage map Mendelian law microsatellite pufferfish Takifugu rubripes |
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