Effect of salinity change on free amino acid content in Pacific oyster |
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Authors: | MASATOMI HOSOI SATOSHI KUBOTA MASAKO TOYOHARA HARUHIKO TOYOHARA and ISAO HAYASHI |
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Institution: | Division of Applied Biosciences,Graduate School of Agriculture, Kyoto University, Kyoto, Kyoto 606-8502,; Department of Agriculture,Kochi University, Nankoku, Kochi 783-8502 and; Departmentof Human Life Studies, Kacho Junior College, Kyoto, Kyoto 605-0062,Japan |
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Abstract: | ABSTRACT: In order to identify free amino acids (FAA) that are importantas intracellular osmolytes in Crassostrea gigas , we investigatedthe change in FAA content in the mantle exposed to an abrupt decreaseor increase in salinity. In hypo-osmotic adaptation, most FAA showedremarkable and synchronous decreases from 2 to 8 h, suggestingthat the non-selective efflux of FAA was mainly responsible forthe decrease in FAA. Taurine that accounted for approximately 80% oftotal FAA content contributed most significantly to the hypo-osmoticadaptation. In hyper-osmotic adaptation, significant increases inglycine, alanine, β-alanine, proline, arginine and taurinewere observed. Of these, alanine showed an immediate increase thatis important to short-term adaptation to hyper-osmolality, whiletaurine showed a slower and substantial increase that contributesto a long-term adaptation to hyper-osmolality. |
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Keywords: | adaptation cell volumeregulation Crassostrea gigas free amino acid mantle osmolyte oyster salinity |
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