突变高温胁迫对大黄鱼血清生理指标的影响

鱼类作为变温动物,在水温突变时体内会发生一系列生理变化。本研究分析了突变高温胁迫下大黄鱼幼鱼血清皮质醇(COR)、血糖(GLU)以及乳酸(BLA)含量的变化规律。实验选取8月龄平均体重为(118.8±6.05)g的大黄鱼作为实验对象,对照组设定温度为(23±0.3)℃,实验组设定温度为(33℃±0.3)℃,变温过程为突变,观察记录实验现象。结果表明:大黄鱼幼鱼在33℃高温胁迫下有强烈的应激反应,表现为呼吸加快、极度不安、游动剧烈。经33℃高温胁迫2 h后,实验组幼鱼血清中皮质醇、血糖、乳酸含量与对照组相比均有显著的升高(P0.05或0.01),陆续出现死亡,最终全部死亡。研究认为,33℃高温胁迫对大黄鱼有致命的影响,在养殖生产过程中要将水温严格控制在33℃以下。

Acute heat stress on the influence of large yellow croaker (Larimichthys crocea) serum physiological indicators

As a cold-blooded animal, fish is particularly sensitive to the changes of the water temperature, and makes rapid response in physiological changes at the same time. This study analyzed the variation law of serum cortisol, blood glucose and lactic acid in the juveniles of large yellow croaker under the stress of muta-tion high temperature. To make it clear, the experiment selected large yellow croaker, the average weight was (118. 8 ± 6. 05) g and 8-month-old as experiment object, and maintained the temperature at (23 ± 0. 3)℃ (the control group) and (33 ± 0. 3)℃ (the test group), temperature-changing process was muta-tion, then we observed and recorded the experimental phenomena. The results indicated that the juveniles of large yellow croaker had a strong stress response, such as accelerating breathing, extreme anxiety, intense swimming, when subjected to high temperature stress at 33℃. The serum of the test group showed a signifi-cant increase in cortisol, glucose, lactic acid content compared to the control group (P<0. 05 or 0. 01) af-ter two hours, and then the juvenile of test group started to die in succession, eventually all died. Research showed that the high temperature of 33℃ stress had a fatal effect on the large yellow croakers, and we should strictly control the water temperature below 33℃ in the actual production.