高温胁迫对棘胸蛙蝌蚪抗氧化酶活力的影响

实验利用水温从18~30℃骤升和缓升两种模式,高温胁迫处理棘胸蛙(Quasipaa spinosa)蝌蚪后,提取其尾部肌肉和内脏团的组织液,进行乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、碱性磷酸酶(AKP)等酶活力和丙二醛(MDA)含量测定。结果表明,棘胸蛙蝌蚪尾部肌肉提取液的LDH活力显著高于内脏团(P0.01);高温胁迫下棘胸蛙蝌蚪尾部肌肉和内脏团提取液的LDH活力显著提高(P0.01)。高温胁迫下棘胸蛙蝌蚪尾部肌肉和内脏团的GSH-Px活力均显著降低(P0.01,P0.05),且水温骤升模式下的内脏团GSH-Px活力显著高于缓升模式(P0.01)。在30℃高温胁迫时,棘胸蛙蝌蚪尾部肌肉和内脏团SOD活力均显著降低(P0.05,P0.01),且水温骤升模式的棘胸蛙蝌蚪尾部肌肉和内脏团SOD活力均高于缓升模式(P0.05,P0.01)。高温胁迫下CAT活力均显著下降(P0.01),且缓升模式下的棘胸蛙蝌蚪CAT活力显著高于骤升模式(P0.05,P0.01)。在两种升温模式下,高温胁迫对尾部肌肉的AKP活力均无显著影响;而在骤升模式下,高温胁迫的棘胸蛙蝌蚪内脏团的AKP活力显著提高(P0.05)。高温胁迫下的棘胸蛙蝌蚪尾部肌肉和内脏团MDA含量均显著升高(P0.01),骤升模式下高温胁迫的棘胸蛙蝌蚪内脏团的MDA含量显著高于尾部肌肉(P0.01)。

High temperature stress on the frog tadpole antioxidant enzyme activity

The activity of LDH, GSH-Px, SOD, CAT, AKP and the content of MDA of the frog tadpole ( Quasipaa spinosa) were detected under raising water temperature from 18℃ to 30℃ with rapid and gentle models. The results showed the activity of LDH extracted from the tail muscle tissue were significantly higher than that of extracted from visceral mass of the frog tadpole (P<0. 01); under heat stress, the activities of LDH extracted from the tail muscle tissue and visceral mass of the frog tadpole were significantly higher ( P<0. 01 ) . The activities of GSH-Px of the tail muscle tissue and visceral mass of the frog tadpole were signifi-cantly lower under heat stress ( P<0. 01 , P<0. 05 ) , and the GSH-Px activities of the visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model signifi-cantly (P<0. 01). Under heat stress, the activities of SOD extracted from the tail muscle tissue and visceral mass of the frog tadpole were significantly lower ( P<0. 05 , P<0. 01 ) , and the SOD activities of the tail muscle tissue and visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model significantly (P <0. 05, P <0. 01). Under heat stress, the activities of CAT extracted from the tail muscle tissue and visceral mass of the frog tadpole were significant drop ( P <0. 01), and under gentle raising temperature model, the activities of CAT extracted from the tail muscle tis-sue of the tadpole were higher than that of rapid raising temperature model significantly ( P <0. 05 , P <0. 01 ) . There were no obvious effects of heat stress on AKP activity of the tadpole tail muscle under the gentle and rapid models of raising water temperature. There were significant rise on AKP activity of the visceral mass of the tadpole under heat stress ( P<0. 05 ) . There were significant rise on MDA contents of the tail and the visceral mass of the tadpole under heat stress ( P<0. 05 ) , and the MDA contents of the visceral mass of the tadpole in the rapid raising temperature model were higher than that of gentle raising temperature model signifi-cantly ( P<0. 01 ) .