| 利用EST—SSR座位对鲤鱼4种生长性状的单标记回归分析 |
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| 引用本文： | 徐美佳,张研,鲁翠云,张晓峰,刘继红,孙效文.利用EST—SSR座位对鲤鱼4种生长性状的单标记回归分析[J].鲑鳟渔业,2009(1):15-18. |
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| 作者姓名： | 徐美佳 张研 鲁翠云 张晓峰 刘继红 孙效文 |
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| 作者单位： | [1]中国水产科学研究院黑龙江水产研究所,农业部水产生物技术重点开放实验室,黑龙江哈尔滨150070 [2]大连水产学院生命科学与技术学院,辽宁大连116023 [3]上海水产大学生命科学与技术学院,上海200090 |
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| 基金项目： | 国家重点基础研究发展计划（编号：2004CB117405）资助项目 |
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| 摘 要： | 获得与经济性状连锁的分子标记或基因是开展该性状分子育种的工作基础。本研究以大头鲤／荷包红鲤抗寒品系的雌核发育群体为试验材料，利用Windows Map Manager2．0的标记回归法进行QTL单标记定位分析，从70个微卫星座位对吻长、头长、尾长、眼间距性状的标记回归研究中，共得到5个标记与性状相关。其中HLJE190、HLJE217、HLJE268与吻长相关，HLJE1与头长、尾长、眼间距相关；C88433与头长、眼间距相关。将C88433通过BLAST比对，结果显示与斑马鱼上控制V型离子泵（ATPase）的基因相关。
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| 关 键 词： | 鲤鱼 EST—SSR 吻长 头长 尾长 眼间距 相关性 |
Analyzing of the EST-SSRs markers related to the 4 growth traits in common carp （Cyprinus carpio L.） by single marker regression |
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| Authors: | XU Mei-jia ZHANG Yan LU Cui-yun ZHANG Xiao-feng LIU Ji-hong SUN Xiao-wen |
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| Institution: | 1. Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. College of Aqu-life Science and Technology, DaLian Fisheries University, DaLian 1160023, China; 3.College of Aqu-life Science and Technology, Shanghai Fisheries University, Shanghai 20090, China) |
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| Abstract: | That gotten molecular markers or genes associated with the economic traits is basic work for marker-assisted breeding. A common carp gynogenetic line of common carp including 44 individuals, was produced from the crosses of Barbless carp × Hebao- cold tolerance red carp, was usedchoosing as mapping panel in this study. Marker regression of Windows Map Manager2.0 software was used to analyze the location of QTLs. Seventy 70 microsatellites loci were employed for the analysis of marker regression on wensizeupper jaw length, headsizehead length, tailsize tail length and eyecross. Three loci （HLJE190,,HLJE217, and HLJE268） related to wensize were discovered related to wensize. Locus HLJE1 were was discovered related to head length, tail length headsize, tailsize and eyecross. C88433 was discovered trlated related to head lengthheadsize and eyecross. C88433 was found associated with the gene which control V-ATPase in zebra fish by using BLAST online in NCBI. |
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| Keywords: | common carp EST-SSR upper jaw lengthwensize head lengthheadsize tail lengthtailsize eyecross correlation analysis |
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