| 线粒体基因组DNA条形码的应用与分析 |
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| 引用本文： | 徐革锋,韩英.线粒体基因组DNA条形码的应用与分析[J].鲑鳟渔业,2013(6):63-67. |
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| 作者姓名： | 徐革锋 韩英 |
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| 作者单位： | [1]中国水产科学研究院黑龙江水产研究所,黑龙江省冷水性鱼类种质资源及增养殖重点开放实验室,中国水产科学研究院冷水性鱼类增养殖生物学与鱼类生理学重点开放实验室,黑龙江哈尔滨150070 [2]东北农业大学动物科技学院水产系,黑龙江哈尔滨150030 |
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| 基金项目： | 农业公益性行业专项（201003055）;中央级公益性科研院所基本科研业务费专项（HSY201312）. |
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| 摘 要： | DNA条形码技术（DNA Barcoding）是一种用短的标准DNA片段快速、准确地识别与鉴定物种的技术。近年来，物种分类和生物多样一直是研究的热点，目前DNA条形码开始广泛应用于生物的遗传多样性研究。本文主要概述了DNA条形码技术的原理、标准基因片段，着重阐述了其在生物分类学和遗传多样性等研究中的应用及其局限性，展望了其发展状况、应用前景和意义。
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| 关 键 词： | DNA条形码 物种分类 标准基因片段 遗传多样性 |
Application and Evaluation of mtDNA Barcoding |
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| Authors: | XU Ge-feng HAN Ying |
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| Institution: | 1. Key Opening Laboratory of Cold-Water Fish Germplasm Resource and Aquaculture ＆ Enhancement, Heilongjiang Province, Key Opening Laboratory of Cold-Water Fish Enhancement Biology ＆ Physiology, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences; Harbin 150070, China; 2. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China) |
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| Abstract: | DNA Barcoding as a method for rapid and accurate species identification based on a standard and short DNA fragments has become one of hotspots of species identification and biodiversity research in the past few years. Currently, DNA barcoding is widely used in genetic diversity as a core issue in biodiversity research. The development, principles, standard gene fragments, and steps of DNA barcodes are described, focusing on the application of DNA barcoding in the study of biological taxonomy and genetic diversity. The research prospects, significance of its application and limitations are discussed. |
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| Keywords: | DNA Barcoding species identification standard gene fi＇agments genetic diversity |
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