南四湖大鳞副泥鳅mtDNAD-loop区遗传多样性分析

根据线粒体D-loop序列研究了南四湖大鳞副泥鳅（Paramisgurnus dabryanus）群体（n=30）的遗传多样性。通过PCR技术对线粒体D-loop序列进行扩增,获得大小约1000 bp的扩增产物。PCR产物经纯化和测序后,得到了963 bp的核苷酸片段。用CLUSTALX软件进行排序比较,在30个个体中,共检测到33个变异位点,包括12个转换位点、21个颠换位点。运用MEGA5.0软件计算出不同个体间的遗传距离,并据此构建了UPGMA与NJ系统树。用DNASP软件计算出的单倍型个数（H）为15,单倍型多样性（h）为0.899,核苷酸多样性（pi）为0.008,平均核苷酸差异数（k）为7.79。结果表明,南四湖大鳞副泥鳅的mtDNA D-loop个体序列变异程度较大,遗传多样性较为丰富。

Genetic diversity analysis of mitochondrial D-loop region of Paramisgurnus dabryanus in Nansi Lake

The PCR technique was used to amplify the mtDNA D-Loop in 30 individuals of Paramisgurnus dabryanus collected from the Nansi Lake.The PCR products were purified and sequenced,and then 963bp nucleotide sequences of partial D-Loop were obtained.CLUSTAL X（1.83） to align and comparison of the sequences of these 30 individuals with each other revealed that there were 33 variation sites including 12 transition sites and 21 transversion sites.The pairwise genetic distances were computed by MEGA,and the UPGMA and NJ phylogenetic trees was established through the cluster analysis over the pairwise genetic distance.DNASP showed that the number of haplotypes（h） was found to be 15,the haplotype diversity（H） 0.899,nucleotide diversity（Pi） 0.008 and the average number of nucleotide diferences（K） 7.79.It can be concluded that the variation in mtDNA D-Loop of Paramisgurnus dabryanus in the Nansi Lake is relatively rich and wealthy on genetic diversity.