金丝桃素、绿原酸和硬葡聚糖对草鱼幼鱼生长及免疫功能的影响

在水温（25±2）℃下，分别在基础饲料中添加不同剂量的硬葡聚糖（0．2％，G1组；0．4％，G2组）绿原酸（0．04％，G3组；0．08％，G4组）和金丝桃素（0．066％，G5组；0．132％，G6组），连续投喂放在1．0m×0．5m×1．0m网箱内、体质量110±5g的草鱼（Ctenopharyngodon idellus）幼鱼35d，以未添加者为对照组，探讨三种添加剂对草鱼幼鱼生长和免疫功能的影响。结果表明，三种添加济对草鱼幼鱼增重率和特定生长率均影响不显著，当饲料中添加0．08％的绿原酸时，草鱼幼鱼末体质量和肝体比显著增加（P〈0．05）；三种添加剂都可显著提高碱性磷酸酶活性，其中以添加0．08％的绿原酸效果最好，添加剂各组间碱性磷酸酶活性差异不显著（P〉0．05）；0．132％金丝桃素组的草鱼血清超氧化物歧化酶活性显著高于对照组，其它试验组与对照组差异不显著；饲料中添加0．2％硬葡聚糖可显著提高草鱼幼鱼血清溶菌酶含量（P〈0．05）；三种添加剂对草鱼幼鱼血清中丙二醛的含量影响不显著（P〉0．05）。

The effects of Hypercin, Chlorogenic acid and Scleroglucan on growth performance and immune function in juvenile grass carp Ctenopharyngodon idellus

Grass carp Ctenopharyngodon idellus juveniles with body weight of 110±5g reared in a net cage of 1.0m×0.5m×1.0m were fed the basal diets supplemented with different levels ofhypericin （0%, 0.066%, and 0.132%）, chlorogenic acid （0%, 0.04%, and 0.08%） and scleroglucan （0%, 0.2%, and 0.4%） at water temperature of 25±2℃ for 35 days to evaluate the effects of the additives on growth performance and immune ftmction of the grass carp juveniles. The results showed that there were no significant differences in weight gain rate and specific growth rate in the juveniles in all experiments （P〉0.05）.However, the juveniles fed the diet containing 0.08% chlorogenic acid had significantly higher final body weight and hepatosomatic index than the juveniles fed the control diet did （P〈0.05）. All of the additives significantly enhanced the activity of alkaline phosphatase （AKP）, the peak value in the fish fed the diet supplemented with 0.08% chlorogenic acid, and no significant differences in AKP activity among all treatments. There was significantly higher activity of superoxide dismutase （SOD） in 0.132% hypericin group than that in the control, whereas there was no significant difference in SOD between the treatments and the control groups. The grass carp juveniles fed the diet supplemented with 0.2% scleroglucan had significantly higher lysozyme activity. The three additives were not found to affect significantly the content of malondialdehyde （MDA） in the serum.