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鱼类消化道内分泌细胞概述及研究方法
引用本文:徐革锋,刘洋,牟振波.鱼类消化道内分泌细胞概述及研究方法[J].鲑鳟渔业,2011(3):60-64,68.
作者姓名:徐革锋  刘洋  牟振波
作者单位:中国水产科学研究院黑龙江水产研究所,哈尔滨150070
基金项目:中央级公益性科研院所基本科研业务费专项(201002 201102); 黑龙江省冷水性鱼类种质资源及增养殖重点开放实验室(201002 2009HSYZX-YZ-03); 中国水产科学研究院冷水性鱼类增养殖生物学与鱼类生理学重点开放实验室(201002 2009HSYZX-YZ-03); 公益性行业(农业)科研专项经费资助(201003055)
摘    要:消化道内分泌细胞能产生多种具有调节胃肠功能的胃肠激素。该类激素除了能促进胃肠对营养物质的消化与吸收外,还能控制摄食行为、调控消化道运动以及细胞营养作用,甚至能够影响其他一些内分泌腺的活动。了解肠道内分泌细胞的基本概况有助于深入地研究鱼类的消化生理,组织化学、免疫细胞化学和电子显微镜等技术是研究消化道内分泌细胞鉴别、定位以及形态学的重要方法,利用这些技术能够揭示鱼类消化道内分泌细胞的生物功能及作用机理,对研究鱼类摄食、消化和吸收等生理机制有重要意义。因此,本文综述了近年来国内外鱼类消化道内分泌细胞的研究进展及主要研究手段,旨在为鱼类消化生理学、内分泌学和营养学提供参考资料。

关 键 词:硬骨鱼类  消化道  内分泌细胞  胃肠激素  组织化学  免疫细胞化学

Current status of research and methods of endocrine cells in digestive tracts in fishes
Authors:XU Ge-feng  LIU Yang  MOU Zhen-bo
Institution:1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China; 2.Department of Biology,College of Life and Environment Science,Harbin Normal University,Harbin 150080,China)
Abstract:Endocrine cell could produced many kinds of gut hormones with the function of regulating stomach and intestine.Besides promoting the digestion and absorption of nutrient substance in stomach and intestine,these hormones could controlled feeding behavior,regulated the movement of digestive tract and trophic action of cell,even influenced the activity of other endocrine glands.Understanding the basic overview of endocrine cell in intestine was conducive to progress the penetrating research on digestive physiology of fishes.Histochemistry immunocytochemistry and electron microscope technique were important ways to study the identification,location and morphology of endocrine cell in digestive tract.Using these techniques could revealed biological function and mechanism of action of endocrine cell in digestive tract,which has meaning to research the physiological mechanism of ingestion,digestion and absorption of fishes.Thus,aimed to offer reference for digestive physiology,endocrinology and nutriology of fishes,we overviewed the progress and main methods in internal and overseas studies of endocrine cell in digestive tract of fishes for the past few years.
Keywords:Osteichthyes  digestive tract  endocrine cell  gut hormone  histochemistry  immunocytochemistry
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