| 二倍体和三倍体虹鳟精液低温保存寿命的研究 |
| |
| 引用本文： | 龚丽,刘奕,杨翟平,郑金霞,范兆廷.二倍体和三倍体虹鳟精液低温保存寿命的研究[J].鲑鳟渔业,2011(1):30-33. |
| |
| 作者姓名： | 龚丽 刘奕 杨翟平 郑金霞 范兆廷 |
| |
| 作者单位： | 东北农业大学动物科技学院,黑龙江哈尔滨150030 |
| |
| 摘 要： | 与二倍体（2n组）精原液做比较,以不同密度为组别,研究了4℃下两组（3n-HD和3n-LD）三倍体虹鳟（Oncorhynchus mykiss）精原液的保存时限。结果发现,随着保存时间的延长,精液中精子的活力和寿命逐渐降低。2n组、3n-HD组和3n-LD组虹鳟精原液中精子分别在保存5d、4.5d和12.5d后失去活力;在7d、15d和14.5 d后死亡,即三倍体虹鳟精原液4℃保存时间约为二倍体的2倍,说明低温短期保存三倍体虹鳟精原液可以取得较好效果。
|
| 关 键 词： | 虹鳟 三倍体精液 低温保存 寿命 |
Cryopreservation of Semen in Diploid-and Triploid Rainbow Trout Oncorhynchus mykiss |
| |
| Authors: | GONG Li LIU Yi YANG Zhaiping ZHENG Jinxia FAN Zhaoting |
| |
| Institution: | (College of Animal Science and Technology,Northeast Agriculture University,Harbin 150030,China) |
| |
| Abstract: | The cryopreservation time of semen was studied in diploid（2n） and triploid rainbow trout（Oncorhunchyus mykiss） at 4 ℃.The results showed that the sperm motility and life span were decreased with the preservation time prolongation.The sperm lost rapid motility after 5 d reservation in group 2n,after 4.5 d reservation in group 3n-HD and after 12.5d reservation in 3n-LD.and was died after 7 d reservation in group 2 n,after 15 d reservation in group 3n-HD and after 14.5 d reservation in 3n-LD.At 4 ℃,the preservation time of semen in the triploid rainbow trout was found to be two times longer than that in the diploid rainbow trout,indicating that the semen of the triploid rainbow trout can be preserved for a short time at 4 ℃. |
| |
| Keywords: | rainbow trout semen of triploids cryopreservation life span |
| 本文献已被 维普 等数据库收录！ |
|
