| 鲤（Cyprinus carpio）Rh糖蛋白家族基因的克隆与组织mRNA表达 |
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| 引用本文： | 董晓丽,位莹莹,徐奇友.鲤（Cyprinus carpio）Rh糖蛋白家族基因的克隆与组织mRNA表达[J].鲑鳟渔业,2013(5):6-10. |
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| 作者姓名： | 董晓丽 位莹莹 徐奇友 |
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| 作者单位： | [1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306 |
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| 基金项目： | 国家大宗淡水鱼类产业技术体系（cARs-46-16）;中央公益性基本科研业务费专项资金（2012NYBsJ-01） |
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| 摘 要： | 为了研究鱼类氨代谢机制，克隆了鲤（Cyprinus carpio）Rh糖蛋白家族的Rhag、Rhbg、Rhcgl基因的cDNA全序列，与人类Rh基因的氨基酸序列比对结果显示，鲤Rh糖蛋白保守性较高。系统分析表明，鲤3个Rh基因与斑马（Daniorerio）Rh基因的亲缘关系较近。Rhag、Rhbg、Rhcgl基因在鲤鳃组织中表达较高，而在其它组织表达的相对量极低。比较3个基因在鳃组织的表达，Rhbg的表达量极显著高于其它2个基因。这一结果预示Rhag、Rhbg、Rhcgl与鲤的氨代谢密切相关，尤其是Rhbg基因，可能是鲤氨代谢的重要因子。
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| 关 键 词： | 鲤 Rh糖蛋白 克隆 表达 |
Cloning and Expression of Rhesus Glycoprotein Genes in Tissues in Common Carp （Cyprinus carpio） |
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| Authors: | DONG Xiao-li WEI Ying-ying ＇ XU Qi-you |
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| Institution: | 1 (1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China; 2. Fisheries and Life Science College, Shanghai Ocean University, Shanghai 201306, China) |
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| Abstract: | In order to study the mechanism of ammonia metabolism in fish, cDNA of three Rhesus glycoprotein genes （Rhag, Rhbg and Rhcgl） were cloned and sequenced in common carp （Cyprinus carpio）, and the amino acids sequences of the three Rh genes was compared with human Rh genes. Phylogenetic tree revealed that the Rh genes of common carp had close relationship with Rh genes of zebra fish （Danio rerio ）. The expressions ofRhag, Rhbg and Rhcgl were found all significantly higher in gill than in any other tissue. In gill tissue, the expression of Rhbg gene was extremely higher than Rhag and Rhcgl gene. These findings indicated that Rhag, Rhbg and Rhcgl gene maybe play an important role in ammonia metabolism in common carp, especially the Rhbg gene while Rhbg gene may be the major transporter of ammonia. |
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| Keywords: | common carp （ Cyprinus carpio） Rhesus glycoprotein cloning expression |
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